Detailed information of TA system TA192218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 5398741..5399360
• Replicon: chromosome
• Accession: NZ_CP069943
• Organism: Klebsiella oxytoca strain FDAARGOS_1332

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: I6K62_RS25110
• Protein ID: WP_004099646.1
• Coordinates: 5399142..5399360 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: I6K62_RS25105
• Protein ID: WP_004099648.1
• Coordinates: 5398741..5399115 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K62_RS25095	5393896..5395089	+	1194	WP_004099653.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
I6K62_RS25100	5395112..5398258	+	3147	WP_004099650.1	multidrug efflux RND transporter permease subunit	-
I6K62_RS25105	5398741..5399115	+	375	WP_004099648.1	Hha toxicity modulator TomB	Antitoxin
I6K62_RS25110	5399142..5399360	+	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
I6K62_RS25115	5399521..5400087	+	567	WP_004099644.1	maltose O-acetyltransferase	-
I6K62_RS25120	5400224..5400694	+	471	WP_004099643.1	YlaC family protein	-
I6K62_RS25125	5400669..5402123	-	1455	WP_017146481.1	PLP-dependent aminotransferase family protein	-
I6K62_RS25130	5402226..5402924	+	699	WP_004099639.1	GNAT family N-acetyltransferase	-
I6K62_RS25135	5402921..5403061	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
I6K62_RS25140	5403061..5403324	-	264	WP_004099638.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T192218 WP_004099646.1 NZ_CP069943:5399142-5399360 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T192218 NZ_CP092184:4647780-4648070 [Serratia marcescens]
ATGATTAAATCTTTCCGCGATCGCTATCTGGAGCAGTTCTATCTGGAAGGCAGGCGCAGCCGTCTGATCCCCGGCGCGCT
TGAGCGACAGTTGGCCAGAAAACTCGATATGTTGGCCGCCGCGCAGCAAGAGCGTGATTTGCATCACCCCACGGGTAACT
ATTACAAGCGGCTATCCGGACCATTTCAGGGATGGTCAAGTCTGCGCGTCAATATGCACTGGCGGCTGATGTTCCAGTGG
CGGCATGATGCCGCTGAAAAAATTTACCTCGATCCTCATCAAGATACCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14350.09 Da
• Isoelectric Point: 4.8989

>AT192218 WP_004099648.1 NZ_CP069943:5398741-5399115 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT192218 NZ_CP092184:4648085-4648408 [Serratia marcescens]
ATGTTGGAAACCACGACTCGCGAACCCACCACGGTGGGAGAAGTGCTGCAGGAAGAATATTTAACGCCGCTAGGCATCGG
CCAGGAGGAACTGGCAAAGGTATTGGGCGTCACCCGCGCCACCGTCAATCGCCTGTGCAACGGGCATCGACGCATGAGCG
TTGCCGAGGCCAATCTGTTTGCCGATCTGTTCGAAACGACCGCTGACTTTTGGCTAAACCTGCAGGCGGCGCACGATCGC
TGGGAAGCCAGACAGGCCGATGCGCAGCGTGTGCGGCTGCGGCCGATCAAAGAAATACTGAGGCGCAATATCACTCATGC
ATGA

Structures

Toxin

• AlphaFold DB: A0A0H3H713