Detailed information of TA system TA192206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 193839..194509
• Replicon: plasmid unnamed
• Accession: NZ_CP069941
• Organism: Klebsiella pneumoniae strain FDAARGOS_1312

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: I6K42_RS26485
• Protein ID: WP_004213072.1
• Coordinates: 193839..194282 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: I6K42_RS26490
• Protein ID: WP_004213073.1
• Coordinates: 194279..194509 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K42_RS26450	189252..189527	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
I6K42_RS26455	189590..190081	+	492	WP_011251327.1	DM13 domain-containing protein	-
I6K42_RS26460	190130..191050	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
I6K42_RS26465	191141..191547	+	407	Protein_204	GAF domain-containing protein	-
I6K42_RS26470	192062..192700	-	639	Protein_205	response regulator	-
I6K42_RS26475	193112..193405	+	294	Protein_206	transposase	-
I6K42_RS26480	193409..193825	+	417	Protein_207	restriction endonuclease	-
I6K42_RS26485	193839..194282	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6K42_RS26490	194279..194509	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6K42_RS26495	195117..196250	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
I6K42_RS26500	196266..196559	+	294	WP_004213076.1	hypothetical protein	-
I6K42_RS26505	196549..196755	-	207	WP_004213077.1	hypothetical protein	-
I6K42_RS26510	197107..197397	+	291	WP_004213078.1	hypothetical protein	-
I6K42_RS26515	197387..198286	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..224781	224781

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T192206 WP_004213072.1 NZ_CP069941:c194282-193839 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T192206 NZ_CP092184:c1118441-1118238 [Serratia marcescens]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT192206 WP_004213073.1 NZ_CP069941:c194509-194279 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT192206 NZ_CP092184:c1118859-1118491 [Serratia marcescens]
ATGGATGAGTATTCGCCTAAGCGGCACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620