Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2558102..2558727 Replicon chromosome
Accession NZ_CP069940
Organism Klebsiella pneumoniae strain FDAARGOS_1312

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag I6K42_RS12240 Protein ID WP_012737091.1
Coordinates 2558344..2558727 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I6K42_RS12235 Protein ID WP_004150355.1
Coordinates 2558102..2558344 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K42_RS12210 2554093..2554692 + 600 WP_004151865.1 glucose-1-phosphatase -
I6K42_RS12215 2554686..2555546 + 861 WP_004146232.1 virulence factor BrkB family protein -
I6K42_RS12220 2555543..2555980 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I6K42_RS12225 2556025..2556966 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I6K42_RS12230 2556980..2557897 - 918 WP_012737090.1 alpha/beta hydrolase -
I6K42_RS12235 2558102..2558344 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I6K42_RS12240 2558344..2558727 + 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K42_RS12245 2558901..2559830 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I6K42_RS12250 2559827..2560462 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I6K42_RS12255 2560459..2561361 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T192199 WP_012737091.1 NZ_CP069940:2558344-2558727 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T192199 NZ_CP092182:c243289-242993 [Alcaligenes faecalis]
ATGATTAAGAGCTTCCGACACAAGGGTCTTCAGGCTTACTTTGAAACCGGCTCCAAATCCGGCATTCAAGCCAAGCATGC
TGTTCGCCTTCAGATTCAGTTAACGGCCCTGCACTCTGCTACTCAAGCGGAAGATATGAACGCCCCCGGATGGAAGCTCC
ACAAGTTATCTGGCAAAAACCCCAAAGGCCAATCTGTACAAGATCACTACACAATATCGGTTAGCGGAAACTGGCGTCTA
ACCTTTTACTTTGAAGGCGAAGACGCTGTTTTGGTGGACTACCAGGACTATCACTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT192199 WP_004150355.1 NZ_CP069940:2558102-2558344 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT192199 NZ_CP092182:c242986-242699 [Alcaligenes faecalis]
ATTATGCGTATGCACAACCCATCCCACCCCGGCCCAGTCTTGCGGGAATACTTGGGCGAACTCAGTGTCACTGAGGCGGC
ATCCCGCCTTGGCGTAACGCGTGCAGCCTTATCGCGCATTCTCAATGGTTCTGCCGGTATTTCGGCAGACATGGCCTTAC
GCCTGCGCGACGCCCTTGGAACCAGTGCCGAAATGTGGATCAACATGCAGTCCCACTACGATCTTTGGCAGGCAGAGCAA
AAGCCAAGGCCCAAAGTCGTGTCCTTTCATGGACCTGCTCATGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References