Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2774514..2775139 Replicon chromosome
Accession NZ_CP069939
Organism Klebsiella pneumoniae strain FDAARGOS_1318

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag I6K48_RS13460 Protein ID WP_004187928.1
Coordinates 2774514..2774897 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I6K48_RS13465 Protein ID WP_004150355.1
Coordinates 2774897..2775139 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K48_RS13445 2771880..2772782 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I6K48_RS13450 2772779..2773414 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I6K48_RS13455 2773411..2774340 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I6K48_RS13460 2774514..2774897 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K48_RS13465 2774897..2775139 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I6K48_RS13470 2775333..2776250 + 918 WP_004187929.1 alpha/beta hydrolase -
I6K48_RS13475 2776264..2777205 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I6K48_RS13480 2777250..2777687 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I6K48_RS13485 2777684..2778544 - 861 WP_004146232.1 virulence factor BrkB family protein -
I6K48_RS13490 2778538..2779137 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T192175 WP_004187928.1 NZ_CP069939:c2774897-2774514 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T192175 NZ_CP092123:c2779659-2779375 [Marinobacterium sediminicola]
ATGACCTATAAAATCAAGTTCATTCCGGCTGCACTGAAAGAATGGAAAAAACTGGCACCGTCATTGCGCGACCAGCTCAA
AAAGAAGCTTTCCGAGCGGGCCGAGAACCCGCATGTACCGGCGTCCCGTCTGCGCGGTTACGAATCGGTTTACAAAATTA
AAATGAAAACTGCCGGGTATCGTTTGGTTTACGAAGTTGAAGACGATGAGATCGTCATCTATGTGCTGGCGGTTGGTAAG
CGTGAAAGAGACGCGGTGTACAAGAAACTTGCAGAGCGACGCTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT192175 WP_004150355.1 NZ_CP069939:c2775139-2774897 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT192175 NZ_CP092123:c2779900-2779649 [Marinobacterium sediminicola]
ATGCAACAGGTGCTTGCCAACTGCTCAGCCAGCATTTCTGAGCTGAAGAAGAATCCCACCGCCCTGTTAAAAGAATCGGG
TGGTGAAACCATCGCCATCCTCAACCACAACAAACCGGCTGCTTACTTGGTGCCTGCCGAAACCTATGAATGGATGATGG
ATCAGCTGGAGGACCAGGCATTGTCTCAGTTGGTTGAGGAACGCAGACAGCAACGCGATCAGGCAATCGAAGTGAGCCTG
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References