Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 25673..26220 Replicon plasmid unnamed2
Accession NZ_CP069936
Organism Escherichia coli strain FDAARGOS_1302

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag I6K32_RS24290 Protein ID WP_001384452.1
Coordinates 25673..25951 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag I6K32_RS24295 Protein ID WP_000079941.1
Coordinates 25951..26220 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K32_RS24260 (21103) 21103..21363 + 261 WP_000343091.1 hypothetical protein -
I6K32_RS24265 (21360) 21360..21950 + 591 WP_001350923.1 hypothetical protein -
I6K32_RS24270 (22012) 22012..22227 - 216 Protein_23 DUF6404 family protein -
I6K32_RS24275 (22356) 22356..22691 + 336 WP_001080729.1 colicin 1A immunity protein -
I6K32_RS24280 (22713) 22713..24593 - 1881 WP_001283335.1 colicin Ia central receptor-binding domain-containing protein -
I6K32_RS24285 (24779) 24779..25627 - 849 WP_001057989.1 3'-5' exonuclease -
I6K32_RS24290 (25673) 25673..25951 - 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
I6K32_RS24295 (25951) 25951..26220 - 270 WP_000079941.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
I6K32_RS24300 (27136) 27136..27993 - 858 WP_000130940.1 incFII family plasmid replication initiator RepA -
I6K32_RS25285 (27986) 27986..28033 - 48 WP_077822033.1 hypothetical protein -
I6K32_RS25290 (28024) 28024..28260 + 237 WP_223368648.1 replication protein RepA -
I6K32_RS24310 (28286) 28286..28546 - 261 WP_000083818.1 replication regulatory protein RepA -
I6K32_RS24315 (28636) 28636..28992 - 357 Protein_33 thermonuclease family protein -
I6K32_RS24320 (29235) 29235..29447 - 213 WP_001311693.1 hypothetical protein -
I6K32_RS24325 (29585) 29585..30145 - 561 WP_000139359.1 fertility inhibition protein FinO -
I6K32_RS24330 (30200) 30200..30946 - 747 WP_000205759.1 conjugal transfer pilus acetylase TraX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA 1..134651 134651


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T192169 WP_001384452.1 NZ_CP069936:c25951-25673 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T192169 NZ_CP092114:105200-105541 [Leptospira noguchii]
TTGGTAAAGAATAGAAACTATACTCCTGAAAAAGGAGATATTGTGTGGCTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAAACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGGTTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAGAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAACTATTAATATTCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT192169 WP_000079941.1 NZ_CP069936:c26220-25951 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT192169 NZ_CP092114:104970-105206 [Leptospira noguchii]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References