Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4244190..4244687 Replicon chromosome
Accession NZ_CP069935
Organism Escherichia coli strain FDAARGOS_1302

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K32_RS20530 Protein ID WP_001173343.1
Coordinates 4244514..4244687 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I6K32_RS20525 Protein ID WP_001312331.1
Coordinates 4244190..4244486 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K32_RS20505 (4240920) 4240920..4241639 - 720 WP_000611291.1 two-component system response regulator DcuR -
I6K32_RS20510 (4241636) 4241636..4243267 - 1632 WP_001216482.1 sensor histidine kinase -
I6K32_RS20515 (4243448) 4243448..4243678 + 231 WP_000373293.1 4Fe-4S mono-cluster protein YjdI -
I6K32_RS20520 (4243690) 4243690..4243962 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6K32_RS20525 (4244190) 4244190..4244486 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K32_RS20530 (4244514) 4244514..4244687 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K32_RS20535 (4244806) 4244806..4246323 - 1518 WP_000003806.1 lysine--tRNA ligase -
I6K32_RS20540 (4246560) 4246560..4248017 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T192160 WP_001173343.1 NZ_CP069935:4244514-4244687 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T192160 NZ_CP092107:1393890-1394420 [Vibrio sp. JC009]
GTGAGCTGGGGAAAAGAGTTTGTAGAGCTAAGTAAATCTGAGCATGACCGCAGCTCTTTTGACTGTGGTGAGCAAGAGCT
TAATACATTTATCAGAACTCAGGCAGCTAAGCATATGCAGGCGGGGATTAGTCGTACTATGATCCTGCCAGGTGTACAGC
CTTTGTTAAATCAAAAGTACGCAATTTGTGCATTTTATAGCATTGCTCCGAGCTCGATAAGTCGTGAAACCCTGCCAAAA
CAGTTGTCGAAAAAGTTACCCGGATATCCTGTTCCCGTATTTTTGTTAGCCCAACTTGCCGTCCATAAGGAGTTTCACGG
TTCAGGTCTTGGAAAAATCAGTTTGATTCGTGCGTTGAAGTATCTATGGGAAATTAATCATCATATGAGAGCTTATGCGA
TTGTTGTTGATTGCCTTACAGACTCGGCTCAGACGTTTTATGCCAAATTTGGTTTTGAGATCTTGTGTGAGCATAACGGG
CGCATTCGTATGTTTTTGCCAATGAAAACTGTCGAACAGATTTTTAATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT192160 WP_001312331.1 NZ_CP069935:4244190-4244486 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT192160 NZ_CP092107:1393624-1393893 [Vibrio sp. JC009]
ATGGCAACTGCACGTTTGGACATCCGCCTTGACGAAGAAATTAAGATAAAGGCAGAAAAAGCATCCGCTTTACTTGGCCT
GAAAAGCCTTACGGAATATGTGGTCAGGCTTATGGATGAAGATGCGACACAGGTTATTGAAGAGCATGAAAGTATTGTCG
TAAAAGATGACGTATTTGATGAGTTTATTGCAGCATGTGATAAAGCGAAGGCTCCAAATCAAGCTCTGCTTGACGCTGCT
AAGTTTGCAGATGAGAGTAATATTAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References