Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2860678..2861479 Replicon chromosome
Accession NZ_CP069935
Organism Escherichia coli strain FDAARGOS_1302

Toxin (Protein)


Gene name yeeV Uniprot ID D8EBK0
Locus tag I6K32_RS13990 Protein ID WP_001094436.1
Coordinates 2861102..2861479 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7MN76
Locus tag I6K32_RS13985 Protein ID WP_015953067.1
Coordinates 2860678..2861055 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K32_RS13945 (2855889) 2855889..2856773 + 885 WP_083574934.1 50S ribosome-binding GTPase -
I6K32_RS13950 (2856815) 2856815..2857270 + 456 Protein_2751 WYL domain-containing protein -
I6K32_RS13955 (2857418) 2857418..2858095 + 678 WP_001097312.1 hypothetical protein -
I6K32_RS13960 (2858101) 2858101..2858334 + 234 WP_001278287.1 DUF905 family protein -
I6K32_RS13965 (2858424) 2858424..2859242 + 819 WP_001175142.1 DUF932 domain-containing protein -
I6K32_RS13970 (2859333) 2859333..2859818 + 486 WP_000860054.1 antirestriction protein -
I6K32_RS13975 (2859833) 2859833..2860309 + 477 WP_001186756.1 RadC family protein -
I6K32_RS13980 (2860378) 2860378..2860599 + 222 WP_000692312.1 DUF987 domain-containing protein -
I6K32_RS13985 (2860678) 2860678..2861055 + 378 WP_015953067.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
I6K32_RS13990 (2861102) 2861102..2861479 + 378 WP_001094436.1 TA system toxin CbtA family protein Toxin
I6K32_RS13995 (2861476) 2861476..2861964 + 489 WP_000761714.1 DUF5983 family protein -
I6K32_RS14000 (2861976) 2861976..2862173 + 198 WP_000839254.1 DUF957 domain-containing protein -
I6K32_RS14005 (2862258) 2862258..2863103 + 846 WP_001280513.1 DUF4942 domain-containing protein -
I6K32_RS14010 (2863174) 2863174..2864709 + 1536 WP_083574935.1 EAL domain-containing protein -
I6K32_RS14015 (2865106) 2865106..2865276 + 171 Protein_2764 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - papG / papF / papE / papK / papJ / papD / papC / papH / papA / papB / papI / kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / neuE / neuC / neuA / neuB / neuD / kpsT / kpsM / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC / rfaE 2818562..3002279 183717
- flank IS/Tn - - 2865172..2865276 104


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13958.84 Da        Isoelectric Point: 6.8603

>T192151 WP_001094436.1 NZ_CP069935:2861102-2861479 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ

Download         Length: 378 bp

>T192151 NZ_CP092106:473544-473834 [Vibrio sp. JC009]
ATGACCTATAAGCTGGATTTTAAAAAGAGTGCTCTCAAAGAGTGGAAAAAGCTGGGCTCGACGTTACAGCAGCAGTTTAA
AAAGAAACTAATTGAGCGTTTGGATAATCCGCATGTACCGGCGTCCAAACTTTCAGGTGCTGATAATTTGTATAAGATTA
AGCTGCGTCAGTCTGGTTATCGTCTGGTGTACAAGGTTGAAGATAACGTAATTGTTGTTACCGTTTTGGCTGTAGGTAAG
CGTGAACGAAGCGATGTTTATCGAAAGGCAATGAAACGTTTGGATGACTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13766.60 Da        Isoelectric Point: 6.2021

>AT192151 WP_015953067.1 NZ_CP069935:2860678-2861055 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Download         Length: 378 bp

>AT192151 NZ_CP092106:473306-473554 [Vibrio sp. JC009]
ATGACCGCTAGAATTTTGGCTGATGTAGCAGCGAGTATTACGGAATTAAAAGCTAATCCAATGAAAGTTGCATCTAGTGC
ATATGGCGAACCTGTTGCCGTGCTAAACCGAAATGAGCCTGCATTTTATTGTGTCCCTGCTGAAGCGTACGAAATGATGA
TGGACAAGCTTGAAGATCTTGAACTGCTTGCTATAGCTCAGGAACGTGAATCTGAAGAGAGTATTTCGGTAAACATTGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2L1N0

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z3CIS0

References