Detailed information of TA system TA192150

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2748778..2749432
• Replicon: chromosome
• Accession: NZ_CP069935
• Organism: Escherichia coli strain FDAARGOS_1302

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: I6K32_RS13400
• Protein ID: WP_000244781.1
• Coordinates: 2748778..2749185 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: I6K32_RS13405
• Protein ID: WP_016239318.1
• Coordinates: 2749166..2749432 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K32_RS13380 (2744735)	2744735..2746468	-	1734	WP_000813190.1	single-stranded-DNA-specific exonuclease RecJ	-
I6K32_RS13385 (2746474)	2746474..2747184	-	711	WP_000715230.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
I6K32_RS13390 (2747209)	2747209..2748105	-	897	WP_000806987.1	site-specific tyrosine recombinase XerD	-
I6K32_RS13395 (2748217)	2748217..2748738	+	522	WP_001055874.1	flavodoxin FldB	-
I6K32_RS13400 (2748778)	2748778..2749185	-	408	WP_000244781.1	protein YgfX	Toxin
I6K32_RS13405 (2749166)	2749166..2749432	-	267	WP_016239318.1	FAD assembly factor SdhE	Antitoxin
I6K32_RS13410 (2749675)	2749675..2750655	+	981	WP_000886078.1	tRNA-modifying protein YgfZ	-
I6K32_RS13415 (2750732)	2750732..2751391	-	660	WP_000250274.1	hemolysin III family protein	-
I6K32_RS13420 (2751555)	2751555..2751866	-	312	WP_001182956.1	N(4)-acetylcytidine aminohydrolase	-
I6K32_RS13425 (2751911)	2751911..2753344	+	1434	WP_001350137.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T192150 WP_000244781.1 NZ_CP069935:c2749185-2748778 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T192150 NZ_CP092106:254156-254419 [Vibrio sp. JC009]
ATGCCGGAAATACTGAAAAGGCTGTTAGGACTTAAGTTCCAGATGAGATATGAGGATAATGACTTTCATAACCGTCCTCA
TATTCATGTTTCTTTTGGTGAGTATGAAGCCTCAATTGCCATCGATGATATCGAAGTACTTGCGGGTTTCCTGCCTCCTA
AAAAGAGGAAGTTGGCAGAATTTTATATTGATAAGCACCGGGACGAGTTATTGCTGGCATGGGACGGAGCGGTAATCGGA
CAAGGTATCGGGAGGATCAATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10573.20 Da
• Isoelectric Point: 5.1599

>AT192150 WP_016239318.1 NZ_CP069935:c2749432-2749166 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDILSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT192150 NZ_CP092106:254419-254934 [Vibrio sp. JC009]
ATGCTTAAAATCGTAGATCTTGACTGGTTGTCCGGCTATCTGCTGGAGCTCGAGTTTAGTGATGGCTTTAAAGGTATTGT
TGATCTGGAAGAGGTTTTTAATTCTGAACCATATAAGCAGATCCAGGATTTTAGCGCCTTTTCACTTGAAGGTACTTATC
TTGACTGGGATGGAGTGGAGCTTTCCGCAAACATTCTTCGAAAAATGGCAGCTGGTAAGGGGCACTACACAGATAGGCCA
TGTGTCAGCGCCAGCAATATGGAAGACGTGTTAAAACAAGCAGCCTGGGATGCGATAGTTTCCAACCGCCCGGATATTTT
TCAGGCCGCAATTAAAGGTTTTGTCGAAGAGTATGGGATTCAGAAAATCCAGCAACACACAAACCTGAAGAGTAGGCCAA
GCATCTACAAGGCATTATCTTCGAACAAGTCTCCAAAACTGGATACGCTCATCCAGTTAGGCCATGCTGCATTCGAGTTA
GAATTTGAGAGTCGAGAAATTAGTCAGAAAGGCTAG

Structures

Toxin

• AlphaFold DB: S1PAM6