Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1727147..1727978 Replicon chromosome
Accession NZ_CP069935
Organism Escherichia coli strain FDAARGOS_1302

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag I6K32_RS08590 Protein ID WP_000854814.1
Coordinates 1727604..1727978 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag I6K32_RS08585 Protein ID WP_001285584.1
Coordinates 1727147..1727515 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K32_RS08555 (1724051) 1724051..1724506 + 456 WP_000581504.1 IrmA family protein -
I6K32_RS08560 (1724585) 1724585..1724818 + 234 WP_001119729.1 DUF905 family protein -
I6K32_RS08565 (1724918) 1724918..1725736 + 819 WP_001234629.1 DUF932 domain-containing protein -
I6K32_RS08570 (1725818) 1725818..1726297 + 480 WP_000860076.1 antirestriction protein -
I6K32_RS08575 (1726313) 1726313..1726789 + 477 WP_001186773.1 RadC family protein -
I6K32_RS08580 (1726852) 1726852..1727073 + 222 WP_000692323.1 DUF987 domain-containing protein -
I6K32_RS08585 (1727147) 1727147..1727515 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
I6K32_RS08590 (1727604) 1727604..1727978 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
I6K32_RS08595 (1727975) 1727975..1728169 + 195 WP_000988600.1 DUF5983 family protein -
I6K32_RS08600 (1728215) 1728215..1728295 + 81 Protein_1696 hypothetical protein -
I6K32_RS08605 (1728584) 1728584..1728712 - 129 Protein_1697 transposase domain-containing protein -
I6K32_RS08610 (1728832) 1728832..1728966 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
I6K32_RS08615 (1729067) 1729067..1729396 - 330 WP_000450409.1 DUF496 family protein -
I6K32_RS08620 (1729568) 1729568..1730626 - 1059 WP_001200888.1 FUSC family protein -
I6K32_RS08625 (1730824) 1730824..1731297 - 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
I6K32_RS08630 (1731416) 1731416..1732582 - 1167 WP_001513842.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T192147 WP_000854814.1 NZ_CP069935:1727604-1727978 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T192147 NZ_CP092104:c1115263-1114862 [Flagellatimonas centrodinii]
ATGTTGCGCTATCTGCTGGACACCAATCTGTGTATCGAGACGATCCGTCACCGGCCGGCGGGGCTCCGTGCCGCTTTCAA
TCGGCATCATGGGCACATGGGGATCAGCGCCGTCACGGTGATGGAGCTGCTGTACGGGGTGGAGCTGTCGGCACAAACGC
AGCGGAACCTCGCAGATGTGGAAGGCTTCATCGCCCGTCTGGATGTGCTGGACTACGATGCGGCCGCTGCTGCGCACACG
GCGCAGATTCGAGCTGAACTGAAAGTCGCCGGCACCCAGATCGGGCCCTATGACCAGATGATTGCCGGCCATGCACGATC
ACGTGGATTGGTGGTGGTGACCAACAACACGAAAGAGTTTCAGCGCGTGGCCGGGCTTCGCATTGAGGACTGGACCACCT
GA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT192147 WP_001285584.1 NZ_CP069935:1727147-1727515 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT192147 NZ_CP092104:c1115493-1115263 [Flagellatimonas centrodinii]
ATGGAACAGGGTGCCGTTTTCAAGAGCAATCGCAGTCAGGCCATTCGGCTGTCCAAGGCGGCGGCGCTGCCGGACGATGT
GAAACGGGTGGATGTGGTGTCGATCGGGCGTACACGCATCATCACGCCGGCCGGAGAGGCCTGGGACAGTTGGTTTGAGG
GTGCCGGCGTCAGCGCCGACTATCTGGCCGAGCGTGAGCAGCCTGCGGCGCAGGAGCGCGATGCGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References