Detailed information of TA system TA192138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1261293..1262881
• Replicon: chromosome
• Accession: NZ_CP069935
• Organism: Escherichia coli strain FDAARGOS_1302

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: I6K32_RS06240
• Protein ID: WP_001125475.1
• Coordinates: 1261293..1262615 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6K32_RS06245
• Protein ID: WP_001332145.1
• Coordinates: 1262615..1262881 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K32_RS06200 (1257079)	1257079..1257312	-	234	WP_000473544.1	hypothetical protein	-
I6K32_RS06205 (1257347)	1257347..1257598	-	252	WP_000381239.1	membrane protein	-
I6K32_RS06210 (1257601)	1257601..1257918	-	318	WP_000073835.1	DNA-binding protein	-
I6K32_RS06215 (1257930)	1257930..1258982	-	1053	WP_000568107.1	phage/plasmid replication protein, II/X family	-
I6K32_RS06220 (1259154)	1259154..1259600	+	447	WP_000527682.1	helix-turn-helix transcriptional regulator	-
I6K32_RS06225 (1259700)	1259700..1260122	+	423	WP_000089313.1	hypothetical protein	-
I6K32_RS06230 (1260234)	1260234..1260701	+	468	WP_000218697.1	hypothetical protein	-
I6K32_RS06235 (1260747)	1260747..1261118	+	372	WP_000795853.1	hypothetical protein	-
I6K32_RS06240 (1261293)	1261293..1262615	-	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6K32_RS06245 (1262615)	1262615..1262881	-	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6K32_RS06250 (1263189)	1263189..1263947	+	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
I6K32_RS06255 (1263951)	1263951..1264865	-	915	WP_001306069.1	bestrophin family protein	-
I6K32_RS06260 (1265062)	1265062..1266513	-	1452	WP_001513806.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T192138 WP_001125475.1 NZ_CP069935:c1262615-1261293 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T192138 NZ_CP092077:c4629133-4628849 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT192138 WP_001332145.1 NZ_CP069935:c1262881-1262615 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT192138 NZ_CP092077:c4629365-4629123 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8