Detailed information of TA system TA192110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 17822..18347
• Replicon: plasmid unnamed6
• Accession: NZ_CP069906
• Organism: Klebsiella pneumoniae strain FDAARGOS_1313

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: I6K43_RS02835
• Protein ID: WP_016946795.1
• Coordinates: 18042..18347 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: I6K43_RS02830
• Protein ID: WP_004197642.1
• Coordinates: 17822..18040 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K43_RS02790	13198..13401	-	204	WP_004197809.1	hypothetical protein	-
I6K43_RS02795	13415..13618	-	204	WP_004197808.1	hemolysin expression modulator Hha	-
I6K43_RS02800	13652..14020	-	369	WP_004197807.1	hypothetical protein	-
I6K43_RS02805	14064..14558	-	495	WP_020802777.1	hypothetical protein	-
I6K43_RS02810	14589..15164	-	576	WP_020317495.1	hypothetical protein	-
I6K43_RS02815	15152..15421	-	270	WP_020804424.1	hypothetical protein	-
I6K43_RS02820	15858..16547	+	690	WP_020315560.1	RES family NAD+ phosphorylase	-
I6K43_RS02825	16579..17268	-	690	WP_004193995.1	hypothetical protein	-
I6K43_RS02830	17822..18040	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
I6K43_RS02835	18042..18347	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
I6K43_RS02840	18505..19188	+	684	WP_023280892.1	hypothetical protein	-
I6K43_RS02845	19191..19967	+	777	WP_049245386.1	site-specific integrase	-
I6K43_RS02850	20025..20282	-	258	WP_000764642.1	hypothetical protein	-
I6K43_RS02855	20411..20515	-	105	WP_032409716.1	hypothetical protein	-
I6K43_RS02860	21050..21916	+	867	WP_004118283.1	replication initiation protein	-
I6K43_RS02865	22273..22542	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..27963	27963
	-	flank	IS/Tn	-	-	12519..13016	497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T192110 WP_016946795.1 NZ_CP069906:18042-18347 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T192110 NZ_CP092072:c2102192-2101983 [Staphylococcus argenteus]
ATGGTACATCAAAACACAATATATACTGCAGGTATTGAAACTGAAGAACAAGTAAACCAGTTGACAGAACGTATATCAAA
CATGATAGGTGTGCATAAAGTGAATATTAATATCATTGATGGAGAAGTAGTTGTAGCATATGAAACACCCGCAAATTTAA
ATAGCATTGAAAAAGAAATTTATGATACAGGCTATAAAATTGTATATTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT192110 WP_004197642.1 NZ_CP069906:17822-18040 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT192110 NZ_CP092072:c2102497-2102204 [Staphylococcus argenteus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATTAAAACAAATCTTAAATCACGTTTAAATCGAATAGAAGGTCA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTTCTTACACAAATAAGAGCGACGCGTTCTG
CATTAAACAGTGTCGCAATAAAGTTATTAGAGCAACATATGAAAAGTTGTATTATGAATAAAGTAAATCAAGGTGCTCAA
GAAGAAGCGATGGAAGAGTTACTAGTTACTTTTCAAAAGTTGATTAAAGATTAA