Detailed information of TA system TA192109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 340275..341041
• Replicon: plasmid unnamed4
• Accession: NZ_CP069904
• Organism: Klebsiella pneumoniae strain FDAARGOS_1313

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: I6K43_RS02630
• Protein ID: WP_032437998.1
• Coordinates: 340550..341041 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: I6K43_RS02625
• Protein ID: WP_162838098.1
• Coordinates: 340275..340562 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K43_RS02610	335423..336661	-	1239	WP_003030308.1	IS110 family transposase	-
I6K43_RS02615	337082..338422	-	1341	WP_000589001.1	ISNCY family transposase	-
I6K43_RS02620	338511..340043	+	1533	WP_015065592.1	IS3-like element ISKpn38 family transposase	-
I6K43_RS02625	340275..340562	+	288	WP_162838098.1	DUF1778 domain-containing protein	Antitoxin
I6K43_RS02630	340550..341041	+	492	WP_032437998.1	GNAT family N-acetyltransferase	Toxin
I6K43_RS02635	341114..341287	+	174	WP_162838097.1	hypothetical protein	-
I6K43_RS02640	341507..341854	-	348	WP_032437997.1	hypothetical protein	-
I6K43_RS02645	342112..343404	-	1293	WP_048268720.1	ABC transporter ATP-binding protein/permease	-
I6K43_RS02650	343392..343865	-	474	WP_129773333.1	hypothetical protein	-
I6K43_RS02655	344596..345081	-	486	WP_204752704.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	pla	1..358127	358127
	-	flank	IS/Tn	-	-	338511..340043	1532

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-89)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17688.45 Da
• Isoelectric Point: 8.5281

>T192109 WP_032437998.1 NZ_CP069904:340550-341041 [Klebsiella pneumoniae]
VGKVTAPVPLSSSHVIAEFHCGEPVLDEWIKQRGLKNQSMGAARTFVVCRENASQVVGFYSLATGSVTHAVAPGSLRRNM
PDPVPVIILARLAVDAGYHGEGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDRARQFYIHHGFIPSQTQERTLFLRLP
VVT

Nucleotide

• Length: 492 bp

>T192109 NZ_CP092072:c2082209-2081847 [Staphylococcus argenteus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTTGT
CATTATTCAAAATGACACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCCA
AAATACCGACCCATGTAGAGATTGAAAAGAAAAAATATAAACTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGG
ACACTTGATAAAAAACGTTTAAAAGAAAAACTGACGTACTTATCAGACAACAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAACACAATGGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10868.54 Da
• Isoelectric Point: 5.1530

>AT192109 WP_162838098.1 NZ_CP069904:340275-340562 [Klebsiella pneumoniae]
MLYTGGSMKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDILDAPVTAN
PAMEKLLARKPRWEK

Nucleotide

• Length: 288 bp

>AT192109 NZ_CP092072:c2082376-2082206 [Staphylococcus argenteus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCAATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA