Detailed information of TA system TA192105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 29356..29999
• Replicon: plasmid unnamed4
• Accession: NZ_CP069904
• Organism: Klebsiella pneumoniae strain FDAARGOS_1313

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: I6K43_RS01080
• Protein ID: WP_046654896.1
• Coordinates: 29356..29772 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: I6K43_RS01085
• Protein ID: WP_039390198.1
• Coordinates: 29769..29999 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K43_RS01065	24691..25575	-	885	WP_048268723.1	LysR family transcriptional regulator	-
I6K43_RS01070	25804..26784	+	981	WP_046654894.1	nitronate monooxygenase	-
I6K43_RS01075	27901..28893	+	993	WP_048268724.1	zinc-dependent alcohol dehydrogenase family protein	-
I6K43_RS01080	29356..29772	-	417	WP_046654896.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6K43_RS01085	29769..29999	-	231	WP_039390198.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6K43_RS01090	30567..30869	+	303	WP_048268725.1	hypothetical protein	-
I6K43_RS01095	30939..31757	+	819	WP_046654910.1	hypothetical protein	-
I6K43_RS01100	31757..32533	+	777	WP_046654911.1	site-specific integrase	-
I6K43_RS01105	32971..34311	-	1341	WP_046654912.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	pla	1..358127	358127
	-	inside	IScluster/Tn	-	-	34459..40814	6355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14921.22 Da
• Isoelectric Point: 7.8881

>T192105 WP_046654896.1 NZ_CP069904:c29772-29356 [Klebsiella pneumoniae]
VNKTYMLDTNICSFIMREQPEAVIKRLEQAVLRNHRIVVSAITYAEMRFGAIGRKAPPRHAQLVDAFCARLDSVLPWDRA
AVDATTEIKATLAAAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWAS

Nucleotide

• Length: 417 bp

>T192105 NZ_CP092070:4992546-4992830 [Pectobacterium sp. PL64]
ATGAGTTATAAGCTGGAGTTTGAAGAGCACGCGCTGAAAGAATTTAAGAAGCTGGGTGCGCCCGTGCGGGAGCAGTTCAC
CATAAAGTTGAAAGAGGTTTTACAAAATCCCCACGTTCCCGCCAACCGACTTCATGGAATGGCTGACTGCTACAAAATCG
AACTTCGTTCCGCTGGCTATCGGTTAGTGTATCAAGTACTTGAGCATGAGATCGTGGTGCTGGTTTTAGCTGTGGGCAAA
CGGGAACGCTCAGACGTTTATAAAACAGCGAAAGACCGACTATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8612.66 Da
• Isoelectric Point: 4.3654

>AT192105 WP_039390198.1 NZ_CP069904:c29999-29769 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVRDGDSIVLRPVRPTWGSFAELEKADAGFMAEREDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT192105 NZ_CP092070:4992305-4992556 [Pectobacterium sp. PL64]
ATGTCCGTGCAACCTATCCTTGCAAATGCAGCCGTGAGTATCAGCGACTTTAAAAAGTCGCCCAATGCCGCGCTTAAAAA
AGCTAACGGCAAACCTGTAGCCGTGCTAACCAATGGCCGTATCTCTGGCTATTATGTTTCCCCGGAAACATGGGAAGCGC
TGGCCGATTATCAGGAAGATATCGAACTTGCCAAAATCGCCCGTTCCCGGATGAAGGGGAAACGTGTGAAGGTCGATCTG
GATGAGTTATAA