Detailed information of TA system TA192104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 85337..85862
• Replicon: plasmid unnamed3
• Accession: NZ_CP069903
• Organism: Klebsiella pneumoniae strain FDAARGOS_1313

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: I6K43_RS00880
• Protein ID: WP_016946795.1
• Coordinates: 85557..85862 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: I6K43_RS00875
• Protein ID: WP_004197642.1
• Coordinates: 85337..85555 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K43_RS00835	80713..80916	-	204	WP_004197809.1	hypothetical protein	-
I6K43_RS00840	80930..81133	-	204	WP_004197808.1	hemolysin expression modulator Hha	-
I6K43_RS00845	81167..81535	-	369	WP_004197807.1	hypothetical protein	-
I6K43_RS00850	81579..82073	-	495	WP_020802777.1	hypothetical protein	-
I6K43_RS00855	82104..82679	-	576	WP_020317495.1	hypothetical protein	-
I6K43_RS00860	82667..82936	-	270	WP_020804424.1	hypothetical protein	-
I6K43_RS00865	83373..84062	+	690	WP_020315560.1	RES family NAD+ phosphorylase	-
I6K43_RS00870	84094..84783	-	690	WP_004193995.1	hypothetical protein	-
I6K43_RS00875	85337..85555	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
I6K43_RS00880	85557..85862	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
I6K43_RS00885	86020..86703	+	684	WP_023280892.1	hypothetical protein	-
I6K43_RS00890	86706..87482	+	777	WP_049245386.1	site-specific integrase	-
I6K43_RS00895	87540..87797	-	258	WP_000764642.1	hypothetical protein	-
I6K43_RS00900	87926..88030	-	105	WP_032409716.1	hypothetical protein	-
I6K43_RS00905	88565..89431	+	867	WP_004118283.1	replication initiation protein	-
I6K43_RS00910	89788..90057	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..95480	95480

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T192104 WP_016946795.1 NZ_CP069903:85557-85862 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T192104 NZ_CP092070:4951431-4951742 [Pectobacterium sp. PL64]
ATGCATGTTATTTCGCGGGCACCTTTCGACACTGCAACCACTCAGTTTCCGAATCAGGCGGCAGCACTTGCTGACCTATA
TCGGGTTATCAAACGCGAAATGTATGCAACGCCGGACGATATGAAAAAACGCTTCCCTAGCCTAGATAGGATGAAATACC
GAGAAAAATGGTGGGTTATTGATATTGGTGGCGGGCATCTTCGAGTGATGTTTTTTGCTGATTTTGAGCGGGGAAAAATC
TTCATCAAGCACATTACATCCCATGCAGAGTACGACAGGCTGACAGAGTATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT192104 WP_004197642.1 NZ_CP069903:85337-85555 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT192104 NZ_CP092070:4951739-4952155 [Pectobacterium sp. PL64]
ATGATGTACGCAGACGCTATCAAGGCAGCTAACAACCTGACGAGTATCGTACCATTCCTCGGAGGTAGCACCTCTCGTAA
GGATTATGAGGATGCGCTGAAACTCACTGAATACCTGATCGAGAGTGAGCCTGATAGTCCGCTAATCGATATGCTGACTG
CCAAAATTGACAAATACGAGAATGATGCACCGGAGTTTGCAGCCTTCAACGAACGTGTTAAAGCACTCCCGGCCGGTATT
GCTTTACTGCGCGTCCTTATGGATCAGCATCAGCTAACGCAGAGTGATTTTGAAGCTGAGATTGGTAAAAAGTCTCTGGT
ATCCCGCATTCTAAACGGTGAGCGCACTCTAACGCTTGATCACATGAGAGCGCTTGCTAAACGATTCGATATTCCTGTGA
GTGCTTTTGTTGATTGA