Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 59760..60496 Replicon plasmid unnamed3
Accession NZ_CP069903
Organism Klebsiella pneumoniae strain FDAARGOS_1313

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag I6K43_RS00745 Protein ID WP_003026803.1
Coordinates 60014..60496 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag I6K43_RS00740 Protein ID WP_003026799.1
Coordinates 59760..60026 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K43_RS00700 55317..55676 - 360 WP_017901436.1 cupin domain-containing protein -
I6K43_RS00705 55827..56216 - 390 WP_017901438.1 DUF86 domain-containing protein -
I6K43_RS00710 56213..56503 - 291 WP_025999361.1 nucleotidyltransferase family protein -
I6K43_RS00715 56516..56773 - 258 WP_204752696.1 hypothetical protein -
I6K43_RS00720 56764..57468 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
I6K43_RS00725 57684..57992 - 309 WP_017896554.1 hypothetical protein -
I6K43_RS00730 58486..59034 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
I6K43_RS00735 59081..59515 + 435 WP_001567367.1 cell envelope integrity protein TolA -
I6K43_RS00740 59760..60026 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
I6K43_RS00745 60014..60496 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
I6K43_RS00750 60697..62100 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
I6K43_RS00755 62129..62761 - 633 WP_001567369.1 hypothetical protein -
I6K43_RS00760 62987..64333 + 1347 WP_077253394.1 ISNCY family transposase -
I6K43_RS00765 64382..64777 + 396 WP_015632387.1 helix-turn-helix domain-containing protein -
I6K43_RS00770 64929..65036 - 108 Protein_74 IS3 family transposase -
I6K43_RS00775 65034..65204 - 171 Protein_75 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..95480 95480
- inside IScluster/Tn - - 48003..67412 19409


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T192103 WP_003026803.1 NZ_CP069903:60014-60496 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T192103 NZ_CP092070:4524073-4525392 [Pectobacterium sp. PL64]
ATGGCTGTGCTCGATGTTTACATGAACGGCTATCGGGTGGGAGTTCTGACCAAAACGGGCAGTGGGGCACATCATTTTAC
CTATGATGCGAACTGGCTCGGATTGCCGGGGAGTCGCCCCATTTCGCTTTCTATGCCGCTACGTCATCAGCCCTATCAAG
GCGATGAAGTTTATAACTTCTTCGACAATTTGTTGCCGGATAACCCGGATATCAGAAGACGGATTGTCGCCAGGCATCAC
GCGGATTCCACCCAACCGTTTGATCTGCTGGCTAAAGTGGGGCAAGACAGTGTCGGGGCGTTGCAATTAGTGCCGCAGGG
TACACCTGTGCACGATATAAAAAAGATCGAATACAAAACCTTATCCGAGCAGCAGCTTGAAAACATATTGACCGGATATC
TGTCCGATGCCCCGCTGGGGATGATTGACGCAGAAGACGATTTTCGTATTTCTATCGCAGGTGCACAGGAAAAGACGGCG
CTGCTTTATCTGGATGATCACTGGTGCCTGCCTAGCAATGCCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATCGAGAGTCACTCTTACTCAATTGATATGTCTGACAGCGTTGAAAATGAGTACCTGTGTTTGCTCATTGCCAAGGCAT
TTGGGCTGCCAGTGCCGCACTGTTTTATGATCAACGCTGGCAAGATAAAGGCGTTGGCGGTGGAGCGTTTTGATCGTAAA
TACGCTTCGGATGTCAGTTGGATTATGCGGCTACCACAAGAGGATTTTTGTCAGGTATTAAACGTTCCTTCCGCACTGAA
ATATGAAAATCATGGTGGACCGGGTATTTCCGCCATTATGTCGTTTCTATTGGGTTCGGTTGATCCAGAAAGAGATCGTT
ATCGCTTTATGCAGGCTCAGGTACTGTTTTGGCTATTGGCTGCAACGGATGGTCATGCGAAAAACTTTTCCCTGTTTATT
GAATCTGAGGGGCGATATCGACTCACGCCTTTCTATGACATTCTCTCTATGTATCCGGCGATGGGCGGACGGGGAATTGA
CCGCAGAGAGGCCAAACTGGCGATGGGATTAACTGGCTCAAGAGGCAAGAAATATGCGATTGAGCAGATCTTTCCCCGAC
ATTTTTTCCAGACGGCGAAGGTCGTTGGGTTTGCCAGAGAATCGATGGAAAACATTCTGGTGGAGTTTGCCCACACCGTT
GAGACCGTCATGACGACTGTTGGAAGCCAACTGCCTGTGGATTTTCCTACTCATATCAGTGAGGCAATTCTGGCCGGGTT
GCAGGCCAGATCGAGACGGCTCATGAGCGGATGGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT192103 WP_003026799.1 NZ_CP069903:59760-60026 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT192103 NZ_CP092070:4523807-4524073 [Pectobacterium sp. PL64]
ATGAAGATCACCAACAGCAAGCAGCTTAGCAGCTATCTGAAAGATGTCCGTATCACGCAGAAGCTTTCACAAGGGAAAGT
GGCCAGTAAAGTGGGTATTCGCCAGGATACGGTATCCAGTTTCGAGCAACATCCTGATTCCACTAAGCTGGAAACCTTCT
TTAAAATCCTGTCGGCATTGAATTTAGAACTTACTGTTACGCCCAGGAATGCGGACAACACCAATAACGAAGCCTCTGTT
GCATCAGCCTGGAAGGAAGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References