Detailed information of TA system TA192102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 117767..118314
• Replicon: plasmid unnamed1
• Accession: NZ_CP069894
• Organism: Escherichia coli strain FDAARGOS_1286

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: I6K16_RS24835
• Protein ID: WP_244582195.1
• Coordinates: 118036..118314 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: I6K16_RS24830
• Protein ID: WP_000079941.1
• Coordinates: 117767..118036 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K16_RS24810 (113000)	113000..113986	-	987	Protein_130	IS4-like element IS421 family transposase	-
I6K16_RS25465 (114167)	114167..114439	-	273	Protein_131	cysteine peptidase family C39 domain-containing protein	-
I6K16_RS24820 (114546)	114546..115820	-	1275	WP_000489607.1	HlyD family secretion protein	-
I6K16_RS24830 (117767)	117767..118036	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
I6K16_RS24835 (118036)	118036..118314	+	279	WP_244582195.1	type II toxin-antitoxin system toxin YacB	Toxin
I6K16_RS24840 (118360)	118360..119208	+	849	WP_001058005.1	3'-5' exonuclease	-
I6K16_RS24845 (119325)	119325..119807	+	483	WP_001311056.1	hypothetical protein	-
I6K16_RS24850 (120109)	120109..120456	+	348	WP_000142437.1	DUF6404 family protein	-
I6K16_RS25470 (120534)	120534..120834	-	301	Protein_139	hypothetical protein	-
I6K16_RS24855 (121015)	121015..121956	+	942	WP_001312841.1	3-deoxy-7-phosphoheptulonate synthase	-
I6K16_RS25475 (121916)	121916..122050	+	135	WP_249531455.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB	1..153221	153221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10746.37 Da
• Isoelectric Point: 7.2749

>T192102 WP_244582195.1 NZ_CP069894:118036-118314 [Escherichia coli]
MEIFWTMLASQDRKCIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTVQKWP

Nucleotide

• Length: 279 bp

>T192102 NZ_CP092070:4377761-4378048 [Pectobacterium sp. PL64]
ATGAGTTATCGCGTCAAATTCAGGGAAGATGCGCTAAAAGAATGGCTCAAGCTCGATAAAACCATTCAGCAGCAATTCGC
TAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTTCGGCAAAGTTACGGGGGATGAAGGATTGCTACAAAATAA
AGCTCCGAGCGTCAGGCTTTCGTTTGGTTTATGAGGTTATTGACGACGTATTGATTATCGCCGTGGTGGCGGTAGGCAAA
CGTGAGCGCAGCGGTGTTTATCATCTGGCGAGTGAGCGGATGAGGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT192102 WP_000079941.1 NZ_CP069894:117767-118036 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT192102 NZ_CP092070:4377513-4377764 [Pectobacterium sp. PL64]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAGCTGAAAAAGAATCCAATGGCGACCGTCAATGCTGG
TGATGGCCTGCCTGTGGCTATCTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAGGAATTAGTTAAGCTGATCGGCGAACGTAAAAACCAGCCGCTGGTTGACGTGGATTTGGAT
AGTTTCCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5