Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 98642..99285 Replicon plasmid unnamed1
Accession NZ_CP069894
Organism Escherichia coli strain FDAARGOS_1286

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag I6K16_RS24715 Protein ID WP_001034044.1
Coordinates 98642..99058 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag I6K16_RS24720 Protein ID WP_001261286.1
Coordinates 99055..99285 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K16_RS24700 (95273) 95273..95970 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
I6K16_RS24705 (95995) 95995..97017 - 1023 WP_000361402.1 helicase UvrD -
I6K16_RS24710 (97002) 97002..98567 - 1566 WP_001128474.1 AAA family ATPase -
I6K16_RS24715 (98642) 98642..99058 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K16_RS24720 (99055) 99055..99285 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
I6K16_RS24725 (99647) 99647..100099 + 453 WP_000670960.1 acyltransferase -
I6K16_RS24730 (100131) 100131..101663 - 1533 WP_001017349.1 NAD(P)-binding domain-containing protein -
I6K16_RS24735 (101660) 101660..101929 - 270 WP_000379710.1 SemiSWEET transporter -
I6K16_RS24740 (101931) 101931..102947 - 1017 WP_000583428.1 Gfo/Idh/MocA family oxidoreductase -
I6K16_RS24745 (102950) 102950..103777 - 828 WP_000154003.1 HAD-IIB family hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB 1..153221 153221


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T192101 WP_001034044.1 NZ_CP069894:c99058-98642 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T192101 NZ_CP092070:c4302664-4301777 [Pectobacterium sp. PL64]
ATGCTGAAGCAACAAATTCGCCAAATTGTCCAGTCAAACCCTGACTATGCCGCAGTGACACCCGTCATAGAAAAAGAGAT
CTTGCATCATGACATTATGGATGTGCTGATCAAACAGGGCGTCATGCAGCGGTTAACGTTCATTGGCGGCACCTCATTAC
GAATGTGTTACAACAGCAGCAGGCTATCAGAAGATCTTGATTTCAATGGTGGTCATGATTTCAAGCCCTCAGCGTTTGAG
GGGCTGGAGTCCGAAATCCAAACCTATATTCAGAACAAGTATGAAACAGAAGTTTGGGTAAACAAACCTTCTGGCGAGCA
GCAAGGGGATACCGTTTCTTGGAAAATTAGTATCGTAAAAGAAGCTAATCGTCCTGATTTGCCACGGCAAAAAATGCATA
TTGACGTCTGTGCTATTCCGTCATTTGATATTGAAAAGCGCCCACTAATCAATCATTACGACATCGTTGTACCGACTGAA
GGGATCTTGGTTCCGGTACAGTCGTTACAAGAAACATTGGCCGATAAGTTGATCGCGTTAGCGTATCGGGCAAGGCGTAT
TAAACCTCGTGATATCTGGGATATCGTTTGGATTAAGCAGCGCGGTATTGATTTATCCAAGACACTGGTTGAAAAGAAGC
TCACCGCCAGACAGAAGCAAACAGAAGACTTTCGACAGGCGCTGTCTATTCAGTTAGCCAAGCTGATGTCGGAAGATGAA
GTCCGTGACGACTTCAATATGGAAATGAGCCGCTTTATCCCTAAGCCAATTAAAGAGAGAACTCTCGATAATCCTGAATA
TTGGGCTTATGTTCAGGGCGAGGTGCATGCCATCGCGTCTGAGTTACTGAATGACGGAGCACCAAAAAATCCATTTGATA
TGGGTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT192101 WP_001261286.1 NZ_CP069894:c99285-99055 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT192101 NZ_CP092070:c4303217-4302693 [Pectobacterium sp. PL64]
ATGGCTAAATCTGATGAGCTATTAAACGCGTTGGCTAAAGCAGTTAACGCTGGCGGTGGCATTCACAGTGTAACAGAGCT
GGCTTTTATGCAGGGTGTGCCTTGTGACTCGGCCTTTAGAAAGTTCCTGGCAGATTGCGTAAAGAAGGGGTTGCTAAGAC
GAGTGGTGAAAGGCTTCTATGAAAGTGTTATTACACCACCTGAGCCCGAGACTGCCATTTATAAAATCATCAAAAAGTTG
CGCAGTGGCACATTGAACTACATCAGCTTAGAAAGTCAGCTCAGTCATACCGGTGATATTTCACAAGTTGTGATGGGTAG
AGTCACTGTGGTAACTAAAGGTCGAAGTGGTTGTTTTGATACGCCTTATGGCGTGATCGAGTTTACCCATACTAAAAAGC
CAGTCGAGCAAATTGCACCTAATTTGTATTATGACCCCGACATCAAAATGTATCGCGCATACAAGGAACAAGCGATTGCA
GATCTAAAACACTGCCAGCGCAATCTACATATGTTGGAGAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References