Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 88742..89277 Replicon plasmid unnamed1
Accession NZ_CP069894
Organism Escherichia coli strain FDAARGOS_1286

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag I6K16_RS24685 Protein ID WP_000222766.1
Coordinates 88990..89277 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag I6K16_RS24680 Protein ID WP_001132900.1
Coordinates 88742..88993 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K16_RS24640 (83869) 83869..84429 + 561 WP_000139315.1 fertility inhibition protein FinO -
I6K16_RS24645 (84565) 84565..84777 + 213 WP_001299730.1 ANR family transcriptional regulator -
I6K16_RS24650 (85023) 85023..85097 + 75 Protein_97 endonuclease -
- (85353) 85353..85414 - 62 NuclAT_1 - -
- (85353) 85353..85414 - 62 NuclAT_1 - -
- (85353) 85353..85414 - 62 NuclAT_1 - -
- (85353) 85353..85414 - 62 NuclAT_1 - -
I6K16_RS24655 (85458) 85458..85607 + 150 WP_001312851.1 Hok/Gef family protein -
I6K16_RS24660 (85891) 85891..86148 + 258 WP_000083845.1 replication regulatory protein RepA -
I6K16_RS25455 (86165) 86165..86416 - 252 WP_223195197.1 replication protein RepA -
I6K16_RS25460 (86407) 86407..86454 + 48 WP_229471593.1 hypothetical protein -
I6K16_RS24670 (86447) 86447..86929 + 483 WP_001273588.1 hypothetical protein -
I6K16_RS24675 (86922) 86922..87779 + 858 WP_000774297.1 incFII family plasmid replication initiator RepA -
I6K16_RS24680 (88742) 88742..88993 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I6K16_RS24685 (88990) 88990..89277 + 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB 1..153221 153221


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T192100 WP_000222766.1 NZ_CP069894:88990-89277 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T192100 NZ_CP092070:c3565800-3565384 [Pectobacterium sp. PL64]
GTGAGTCGCTTGTTCATGCTGGATACCAATATCTGTTCCTTCATTATGCGTGAACACCCGACAGCGGTGATTGACAAGTT
ACAGCAGTGCACATTGCGGCGTGATCGGATCGTGGTCTCTGCCATCACTTACGCTGAAATGCGTTTCGGGGCGGTTGGAA
AAAAAGCCTCGCCACGCCATATGCAACTAGTTGATGCTTTCTGTCGTCGGCTCGATGCGGTGTTACCTTGGGATAGCGCA
GCAGTAGACGCTACTACGAAAATCAAAATTGCGTTAGCCGCCGCAGGCACGCCTATTGGCCCAAATGATACGGCCATCGC
TGGTCATGCCATCAGCGCTAAGGCCATATTGGTTACCAATAATCTGAGAGAGTTTGAAAGAGTGCAGGGGCTGACGTTGG
AAGATTGGGTTAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT192100 WP_001132900.1 NZ_CP069894:88742-88993 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT192100 NZ_CP092070:c3566024-3565797 [Pectobacterium sp. PL64]
ATGCGTATTGTTTCGGTATTTAAAAACGGTAACAACCGTGCCATTCGCTTGCCGCGTGATATGGACTTTGAAGGGGTCAA
CGAACTGGAGATCGTGCGTGAGGGGGATACCATCATGCTGCGTCCGGTTAAACCCAATTGGCATTCATTTTTGCAAGAAG
AGCGCGCTGACGCTGACTTTATGACAGAACGTCAGGATATAGTCGAAGAGGGGAGATTCGAGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References