Detailed information of TA system TA192096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 76104..76729
• Replicon: plasmid unnamed1
• Accession: NZ_CP069894
• Organism: Escherichia coli strain FDAARGOS_1286

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6K16_RS24615
• Protein ID: WP_000911333.1
• Coordinates: 76104..76502 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: I6K16_RS24620
• Protein ID: WP_000450520.1
• Coordinates: 76502..76729 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K16_RS24600 (72435)	72435..72908	+	474	WP_204669886.1	conjugal transfer entry exclusion protein TraS	-
I6K16_RS24605 (72958)	72958..73689	+	732	WP_000850422.1	conjugal transfer complement resistance protein TraT	-
I6K16_RS24610 (73942)	73942..76095	+	2154	WP_204669888.1	type IV conjugative transfer system coupling protein TraD	-
I6K16_RS24615 (76104)	76104..76502	-	399	WP_000911333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
I6K16_RS24620 (76502)	76502..76729	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB	1..153221	153221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14919.19 Da
• Isoelectric Point: 7.8605

>T192096 WP_000911333.1 NZ_CP069894:c76502-76104 [Escherichia coli]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T192096 NZ_CP092070:c972005-971586 [Pectobacterium sp. PL64]
ATGACAGATATCTTACTCAACAAAAGCAGCACCATACAAAGATGCCTACGTCGTATCCGTGAGGAATTCGATACTGAGGA
AGGGTTTCGTCAAAACTTCACCAAGCAAGATTCTGTCATACTCAACATACAACGCGCCTGCGAAGCCGCTATTGATATCG
CTAATTATCTTATCCGTATCAAGCAGCTAGGTATACCACAGAGTAGCCGCGACAGCTTCGCGCTTCTTGCTGCAAACCAG
ATTATTACGATTGATCTGTCTGATAATTTGCAAAAAATGGTGGGATTAAGAAACATTGCCGTGCATGACTATCAGGCGCT
CAATTTGGATATTGTCATCCACGTCATTTCTCATCGTTTAAGCGACTTTGAGCGTTTTATCAAAGAAATAGCCCGATACA
GCGACAGCCAGCACCTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT192096 WP_000450520.1 NZ_CP069894:c76729-76502 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT192096 NZ_CP092070:c972385-972002 [Pectobacterium sp. PL64]
ATGTTCGATGAGATCGTTTTGACACTGAAAAAACAGATGCCTGACATCAAGATGATTTACCTCTTTGGTTCCCAGGCAAC
AGGCAATGCCAGAGCAGACAGCGACGTTGATATCGCAATCATGGCTACGAGAGCCTTGGATCCCGTTGAACGCTGGGAGC
TCTCCCACCAGTTAGCAAAAACAGTAGGTCATGATGTCGATCTTATCGATTTATTGCAGGCCTCGACGGTATTAAAAATG
GAGATCGTTCGTAACGGCAAGCTACTTTACGATGCGGAAGCAACAGCGGGAGAATTTGAAATGACAACACTTTCGATGTA
CCAGCATCTGCAAAAAGAGCGTGCGGATATCATTCGTAGCTTTAATCAGGATCTAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2