Detailed information of TA system TA192091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4879108..4879744
• Replicon: chromosome
• Accession: NZ_CP069893
• Organism: Escherichia coli strain FDAARGOS_1286

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: I6K16_RS23645
• Protein ID: WP_000813796.1
• Coordinates: 4879108..4879284 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6K16_RS23650
• Protein ID: WP_076838470.1
• Coordinates: 4879328..4879744 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K16_RS23625 (4874730)	4874730..4875902	-	1173	WP_001236216.1	BenE family transporter YdcO	-
I6K16_RS23630 (4875994)	4875994..4876530	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
I6K16_RS23635 (4876603)	4876603..4878564	+	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I6K16_RS23640 (4878656)	4878656..4878886	-	231	WP_000494243.1	YncJ family protein	-
I6K16_RS23645 (4879108)	4879108..4879284	+	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I6K16_RS23650 (4879328)	4879328..4879744	+	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I6K16_RS23655 (4879823)	4879823..4881229	+	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
I6K16_RS23660 (4881474)	4881474..4882619	+	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
I6K16_RS23665 (4882637)	4882637..4883650	+	1014	Protein_4662	ABC transporter ATP-binding protein	-
I6K16_RS23670 (4883651)	4883651..4884592	+	942	WP_001251307.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T192091 WP_000813796.1 NZ_CP069893:4879108-4879284 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T192091 NZ_CP092065:c3556211-3555834 [Edwardsiella ictaluri]
ATGGGCAAGAACTTGCGAAAACATAACAATATTGTTATGTTTCACTCCATGAACTACACTATTGAGTACTACAGCGAAGA
GGTTCGGCTTGAGGTCGATCAGTTGCCAATGGGTATGCGGGTTCGATACCAGCATCTCGTTGAACGCATGGAAATCTACG
GTAGTAATCTCGGAGAACCACACACAAGCCCTTTTGGCGATGGGCTTTTTGAGCTTCGAATCAAAGGCAGTGATGGCATC
GCCCGCGTGTTTTACTGCACTCTTACCGGAAAACGTATCGTCATGCTGCACAGCTTCATCAAGAAAACCCAGAAGACGCC
AAGCGCTGAACGTAAGAAGGCTGAAACCAGAATGAAGGAGGTAAAACATGGCTGGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT192091 WP_076838470.1 NZ_CP069893:4879328-4879744 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT192091 NZ_CP092065:c3555818-3555537 [Edwardsiella ictaluri]
ATGACGCATGATGAAATGGCTGCAAAATGGATGGAAGACCCGGCATTTAAAGCAGAATACGATGCCATCGAAGAGGAATA
CGCCCTACTCGATGAAATGCTCGCAGCCCGCAAAAACGCGGGGCTGACCCAGGCGCAGGTCGCCGAACGTATGGGAACCA
AGGCTACAGCGATCACTCGCATGGAAAGTAATTTAGCCTCCGGCCAAAGCGGGCCATCATTCGCCACGCTGAAAAAATTT
GCACACGCGACCGGCAAGAAACTGCAGATCCGGTTTGTCTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3