Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3823403..3824021 Replicon chromosome
Accession NZ_CP069893
Organism Escherichia coli strain FDAARGOS_1286

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K16_RS18230 Protein ID WP_001291435.1
Coordinates 3823403..3823621 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K16_RS18235 Protein ID WP_000344800.1
Coordinates 3823647..3824021 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K16_RS18195 (3818692) 3818692..3819264 + 573 WP_000779821.1 YbaY family lipoprotein -
I6K16_RS18200 (3819295) 3819295..3819606 - 312 WP_000409911.1 MGMT family protein -
I6K16_RS18210 (3819985) 3819985..3820338 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K16_RS18215 (3820380) 3820380..3821930 - 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K16_RS18220 (3822094) 3822094..3822564 - 471 WP_000136192.1 YlaC family protein -
I6K16_RS18225 (3822680) 3822680..3823231 - 552 WP_000102564.1 maltose O-acetyltransferase -
I6K16_RS18230 (3823403) 3823403..3823621 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K16_RS18235 (3823647) 3823647..3824021 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K16_RS18240 (3824567) 3824567..3827716 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
I6K16_RS18245 (3827739) 3827739..3828932 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192087 WP_001291435.1 NZ_CP069893:c3823621-3823403 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192087 NZ_CP092065:577077-577280 [Edwardsiella ictaluri]
ATGACAAAAATCGATTATCTGATGAAGTTGCGTAAATGCACGACGCTGGATACTCTGGAACGCGTGATCGAAAAGAACAA
ATACGAACTTTCAGATGATGAGCTGGAAATTTTTTATTCTGCCGCAGATCATCGACTGGCTGAATTAACCATGAATAAAC
TATACGACAAAATTCCGGCAGAAGTTTGGCAATACGTCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192087 WP_000344800.1 NZ_CP069893:c3824021-3823647 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192087 NZ_CP092065:576688-577056 [Edwardsiella ictaluri]
ATGGATGAGAACACTTCTTATCAGCATGACATCTCAGAGTTAAAATACCTGTGTGATTACCTGTATCATCAGGGAATAGA
TGTCTTGGGAGAAAGCAATCACGGCTGGGTCAGCGATCCGACGGCAGAAGTAAATCTACAGCTTAACGAGCTGATTGAGC
ATATTGCCAGCATCGCGCAATCATTTAAGATCAAATATCCGCGTCATTCGGATTTAGCCGAAATGCTGGATTATTATCTG
GATGAAACCTATGCCCTGTTTGGCACCTATAGCATAAGTGAAACGGCGCTCAGGCAATGGTTGCGTACCAAACGTCGGAT
GGCATACTGTCTGGCGCATGAAAAGCGCAACGCTGCCTTACATGTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References