Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1523078..1523732 Replicon chromosome
Accession NZ_CP069893
Organism Escherichia coli strain FDAARGOS_1286

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I6K16_RS07320 Protein ID WP_000244781.1
Coordinates 1523078..1523485 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K16_RS07325 Protein ID WP_000354046.1
Coordinates 1523466..1523732 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K16_RS07300 (1519035) 1519035..1520768 - 1734 WP_000813191.1 single-stranded-DNA-specific exonuclease RecJ -
I6K16_RS07305 (1520774) 1520774..1521484 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K16_RS07310 (1521509) 1521509..1522405 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6K16_RS07315 (1522517) 1522517..1523038 + 522 WP_001055874.1 flavodoxin FldB -
I6K16_RS07320 (1523078) 1523078..1523485 - 408 WP_000244781.1 protein YgfX Toxin
I6K16_RS07325 (1523466) 1523466..1523732 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K16_RS07330 (1523985) 1523985..1524965 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
I6K16_RS07335 (1525042) 1525042..1525701 - 660 WP_000250269.1 hemolysin III family protein -
I6K16_RS07340 (1525865) 1525865..1526176 - 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
I6K16_RS07345 (1526221) 1526221..1527654 + 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T192073 WP_000244781.1 NZ_CP069893:c1523485-1523078 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T192073 NZ_CP092052:c2486515-2486408 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT192073 WP_000354046.1 NZ_CP069893:c1523732-1523466 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT192073 NZ_CP092052:2486331-2486391 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References