Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 25732..26257 Replicon plasmid unnamed
Accession NZ_CP069891
Organism Escherichia coli strain FDAARGOS_1295

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag I6K25_RS24280 Protein ID WP_042191934.1
Coordinates 25952..26257 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6K25_RS24275 Protein ID WP_000813634.1
Coordinates 25732..25950 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K25_RS24270 (22769) 22769..23410 - 642 WP_000990667.1 hypothetical protein -
I6K25_RS24275 (25732) 25732..25950 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6K25_RS24280 (25952) 25952..26257 + 306 WP_042191934.1 type II toxin-antitoxin system toxin CcdB Toxin
I6K25_RS24285 (26258) 26258..27067 + 810 WP_000016979.1 site-specific integrase -
I6K25_RS24290 (27205) 27205..27480 - 276 WP_000239529.1 hypothetical protein -
I6K25_RS24295 (27474) 27474..28118 - 645 WP_000633911.1 AAA family ATPase -
I6K25_RS24300 (28347) 28347..29318 + 972 WP_001103694.1 plasmid segregation protein ParM -
I6K25_RS24305 (29323) 29323..29715 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
I6K25_RS24310 (29720) 29720..30990 - 1271 Protein_32 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..114230 114230


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11682.45 Da        Isoelectric Point: 6.4674

>T192062 WP_042191934.1 NZ_CP069891:25952-26257 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTSGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T192062 NZ_CP092042:c4869933-4869622 [Enterobacter cloacae]
ATGCTATTCATTGAAACGGATATCTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATACCATAAGCTCCAGGT
GTTCCTGGCAACACAACCTGATTATGGCGATCTGATCCAGAATACCGGGGGGCTGCGAAAGATTCGTTGGCTTTCAGGAG
GCAGGGGAAAGCGCGGTGGAGTAAGGGTTATTTATTTTCATCGTACCCACGAATTTGAAATCAGATTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTGTCCGCGAGAGAAAAAGCCATTCTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT192062 WP_000813634.1 NZ_CP069891:25732-25950 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT192062 NZ_CP092042:c4869621-4869331 [Enterobacter cloacae]
ATGGATAAGGCACTGTTTGAGCGATTAACACAAAGCATAGCTCAGATGGATGACATTGTCGGGGGGAAACGCGAGCCGTC
GCGCACCTTTCAAATCGATGCGATGAAAATCAAAGAAATTCGTCAGGCGTCTGGGTTGTCCCAAAGTAAATTCGCGAACT
TAATCTCAGTCAGCGTTGATACGTTGCGTAACTGGGAACAAGGACGACGTTCGCCTACCGGTCCCGCAAAGGCATTGTTG
CGTGCAATTGCGAACGATCCACAGCACGTTATTTCGGCTTTATCAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References