Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3176099..3176717 Replicon chromosome
Accession NZ_CP069890
Organism Escherichia coli strain FDAARGOS_1295

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K25_RS15155 Protein ID WP_001291435.1
Coordinates 3176099..3176317 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K25_RS15160 Protein ID WP_000344800.1
Coordinates 3176343..3176717 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K25_RS15120 (3171386) 3171386..3171958 + 573 WP_000779831.1 YbaY family lipoprotein -
I6K25_RS15125 (3171989) 3171989..3172300 - 312 WP_000409908.1 MGMT family protein -
I6K25_RS15135 (3172679) 3172679..3173032 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K25_RS15140 (3173074) 3173074..3174624 - 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K25_RS15145 (3174788) 3174788..3175258 - 471 WP_000136192.1 YlaC family protein -
I6K25_RS15150 (3175374) 3175374..3175925 - 552 WP_000102543.1 maltose O-acetyltransferase -
I6K25_RS15155 (3176099) 3176099..3176317 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K25_RS15160 (3176343) 3176343..3176717 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K25_RS15165 (3177262) 3177262..3180411 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
I6K25_RS15170 (3180434) 3180434..3181627 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192043 WP_001291435.1 NZ_CP069890:c3176317-3176099 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192043 NZ_CP092040:c3615735-3615322 [Salmonella enterica subsp. enterica serovar Infantis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTTCTGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192043 WP_000344800.1 NZ_CP069890:c3176717-3176343 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192043 NZ_CP092040:c3615982-3615716 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References