Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2567898..2568733 Replicon chromosome
Accession NZ_CP069890
Organism Escherichia coli strain FDAARGOS_1295

Toxin (Protein)


Gene name yeeV Uniprot ID Q1R2L4
Locus tag I6K25_RS12320 Protein ID WP_001094426.1
Coordinates 2568356..2568733 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID Q1R2L5
Locus tag I6K25_RS12315 Protein ID WP_001285575.1
Coordinates 2567898..2568266 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K25_RS12280 (2563791) 2563791..2564471 + 681 WP_001282919.1 WYL domain-containing protein -
I6K25_RS12285 (2564619) 2564619..2565296 + 678 WP_001097301.1 hypothetical protein -
I6K25_RS12290 (2565302) 2565302..2565535 + 234 WP_001278290.1 DUF905 family protein -
I6K25_RS12295 (2565625) 2565625..2566443 + 819 WP_001175160.1 DUF932 domain-containing protein -
I6K25_RS12300 (2566534) 2566534..2567019 + 486 WP_000213697.1 antirestriction protein -
I6K25_RS12305 (2567034) 2567034..2567510 + 477 WP_001298074.1 RadC family protein -
I6K25_RS12310 (2567597) 2567597..2567818 + 222 WP_000692350.1 DUF987 domain-containing protein -
I6K25_RS12315 (2567898) 2567898..2568266 + 369 WP_001285575.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
I6K25_RS12320 (2568356) 2568356..2568733 + 378 WP_001094426.1 TA system toxin CbtA family protein Toxin
I6K25_RS12325 (2568730) 2568730..2569218 + 489 WP_000761683.1 DUF5983 family protein -
I6K25_RS12330 (2569230) 2569230..2569427 + 198 WP_000839272.1 DUF957 domain-containing protein -
I6K25_RS12335 (2569512) 2569512..2569661 + 150 Protein_2421 hypothetical protein -
I6K25_RS12340 (2570889) 2570889..2572652 + 1764 WP_001555776.1 hypothetical protein -
I6K25_RS12345 (2573051) 2573051..2573230 - 180 Protein_2423 peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE / fimA / fimI / fimC 2555195..2582789 27594


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14072.99 Da        Isoelectric Point: 7.3523

>T192039 WP_001094426.1 NZ_CP069890:2568356-2568733 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T192039 NZ_CP092040:c1109990-1109772 [Salmonella enterica subsp. enterica serovar Infantis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCTGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13709.52 Da        Isoelectric Point: 6.6258

>AT192039 WP_001285575.1 NZ_CP069890:2567898-2568266 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT192039 NZ_CP092040:c1110392-1110018 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454ABD6

Antitoxin

Source ID Structure
AlphaFold DB A0A454ABF5

References