Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2302073..2302570 Replicon chromosome
Accession NZ_CP069890
Organism Escherichia coli strain FDAARGOS_1295

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K25_RS11065 Protein ID WP_001173343.1
Coordinates 2302397..2302570 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I6K25_RS11060 Protein ID WP_001312331.1
Coordinates 2302073..2302369 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K25_RS11040 (2298802) 2298802..2299521 - 720 WP_000611291.1 two-component system response regulator DcuR -
I6K25_RS11045 (2299518) 2299518..2301149 - 1632 WP_001216482.1 sensor histidine kinase -
I6K25_RS11050 (2301330) 2301330..2301560 + 231 WP_000373293.1 4Fe-4S mono-cluster protein YjdI -
I6K25_RS11055 (2301572) 2301572..2301844 + 273 WP_000405646.1 GNAT family N-acetyltransferase -
I6K25_RS11060 (2302073) 2302073..2302369 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K25_RS11065 (2302397) 2302397..2302570 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K25_RS11070 (2302689) 2302689..2304206 - 1518 WP_000003806.1 lysine--tRNA ligase -
I6K25_RS11075 (2304443) 2304443..2305900 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T192038 WP_001173343.1 NZ_CP069890:2302397-2302570 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T192038 NZ_CP092040:c909322-909065 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCAGTTACTCACATATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCGCAGCGTGACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAACAGCTTTACAAGGCTGAACAGGTGGTTAAGGGGATG
CGGGACGTGATTGTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT192038 WP_001312331.1 NZ_CP069890:2302073-2302369 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT192038 NZ_CP092040:909147-909479 [Salmonella enterica subsp. enterica serovar Infantis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTCACGCTGCGCCCGGTCTCAAATCCCGCTTCTTCCTGCCA
GTCACCCTTAAGGTGCTGGCTGGGGATATGTGAGTAACTGGTGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCTCGGCCAGCACCGGGCGGTGATGAGCAACCGGCTACC
GGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References