Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 848961..849615 Replicon chromosome
Accession NZ_CP069890
Organism Escherichia coli strain FDAARGOS_1295

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I6K25_RS04070 Protein ID WP_000244781.1
Coordinates 848961..849368 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K25_RS04075 Protein ID WP_000354046.1
Coordinates 849349..849615 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K25_RS04050 (844918) 844918..846651 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
I6K25_RS04055 (846657) 846657..847367 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K25_RS04060 (847392) 847392..848288 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
I6K25_RS04065 (848400) 848400..848921 + 522 WP_001055874.1 flavodoxin FldB -
I6K25_RS04070 (848961) 848961..849368 - 408 WP_000244781.1 protein YgfX Toxin
I6K25_RS04075 (849349) 849349..849615 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K25_RS04080 (849858) 849858..850838 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
I6K25_RS04085 (850915) 850915..851574 - 660 WP_000250274.1 hemolysin III family protein -
I6K25_RS04090 (851738) 851738..852049 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I6K25_RS04095 (852094) 852094..853527 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T192035 WP_000244781.1 NZ_CP069890:c849368-848961 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T192035 NZ_CP092039:4884487-4884774 [Pectobacterium brasiliense]
ATGAGCTATAGCGTTAAATTCAGGGAAGATGCGCTGAAAGAATGGCTCAAGCTCGATAAAACCATCCAGCAGCAATTTGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTTCAGCAAAGTTACGGGGGATGAAGGACTGCTACAAAATAA
AGCTCCGAGCGTCAGGCTTTCGTTTGGTTTATGAGGTTATTGACGACGTATTGATTATCGCCGTAGTGGCGGTAGGTAAA
CGTGAGCGCAGCGGTGTTTATCATCTGGCGAGTGAGCGAATGAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT192035 WP_000354046.1 NZ_CP069890:c849615-849349 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT192035 NZ_CP092039:4884239-4884490 [Pectobacterium brasiliense]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAACTGAAAAAGAACCCGATGGCGACGGTCAATGCTGG
CGATGGCCTGCCTGTTGCTATCTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAAGAACTGGTTAAGCTAATCGGTGAACGCAAAAACCAGCCGCTGGTTGACGTGGATTTGGAT
AGTTTCCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References