Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 62161..62762 Replicon plasmid unnamed3
Accession NZ_CP069885
Organism Escherichia coli strain FDAARGOS_1289

Toxin (Protein)


Gene name doc Uniprot ID U9ZW91
Locus tag I6K19_RS26075 Protein ID WP_001216033.1
Coordinates 62382..62762 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag I6K19_RS26070 Protein ID WP_001190712.1
Coordinates 62161..62382 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K19_RS27280 57433..57681 + 249 Protein_73 hypothetical protein -
I6K19_RS27285 57775..57960 + 186 WP_231532152.1 DUF551 domain-containing protein -
I6K19_RS26025 (I6K19_26025) 57957..58217 + 261 WP_000982275.1 hypothetical protein -
I6K19_RS26030 (I6K19_26030) 58303..58536 + 234 WP_000516537.1 hypothetical protein -
I6K19_RS26035 (I6K19_26035) 58715..59008 + 294 WP_000269004.1 hypothetical protein -
I6K19_RS26040 (I6K19_26040) 59015..59389 + 375 WP_000988658.1 hypothetical protein -
I6K19_RS26045 (I6K19_26045) 59371..60303 + 933 WP_077787973.1 hypothetical protein -
I6K19_RS26050 (I6K19_26050) 60300..60662 + 363 WP_001261544.1 hypothetical protein -
I6K19_RS26055 (I6K19_26055) 60737..60874 - 138 WP_000123562.1 hypothetical protein -
I6K19_RS26060 (I6K19_26060) 61324..61575 + 252 WP_046644566.1 DNA polymerase III subunit theta -
I6K19_RS26065 (I6K19_26065) 61699..62088 + 390 WP_000506726.1 S24 family peptidase -
I6K19_RS26070 (I6K19_26070) 62161..62382 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I6K19_RS26075 (I6K19_26075) 62382..62762 + 381 WP_001216033.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
I6K19_RS26080 (I6K19_26080) 62767..62946 + 180 WP_001339207.1 hypothetical protein -
I6K19_RS26085 (I6K19_26085) 62974..64017 + 1044 WP_000648833.1 DUF968 domain-containing protein -
I6K19_RS26090 (I6K19_26090) 64106..64558 + 453 WP_001326849.1 late promoter-activating protein -
I6K19_RS26095 (I6K19_26095) 64644..65837 + 1194 WP_000219618.1 hypothetical protein -
I6K19_RS26100 (I6K19_26100) 65837..67321 + 1485 WP_000124159.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..95822 95822


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13571.31 Da        Isoelectric Point: 5.6343

>T192033 WP_001216033.1 NZ_CP069885:62382-62762 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Download         Length: 381 bp

>T192033 NZ_CP092039:c4479459-4479184 [Pectobacterium brasiliense]
ATGGAAATACGTTGGACGCAAAAGGCACAGGATGACCTGGAACGTATTTATGGGTTTGCCAGCCAGTACAGTCGGCAACA
TGCTGACGAGGTGTTAGATCGTTTGATCATCGGCACCACGGGGCTTGCTGACTACCCGAGAATCGGCGTATCTCAGACAA
GGTATGAACCACGTGAGGTGAGAAAGATTTTGTTTGATGATTATGAAGTTCACTATGAAATTCAGCATGATACGAGTTAT
ATCGTCGATCTGTGGCATAAACGAGAAGACCGCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT192033 WP_001190712.1 NZ_CP069885:62161-62382 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT192033 NZ_CP092039:c4479711-4479463 [Pectobacterium brasiliense]
ATGGGACAACCCGCGAAACAGACGATCAGTGCTCAGATACCCGCCGAACTTGCGGCTGCGGTTGAAAGACTGGCTATTGA
ATTGGATAGATCCAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCAATGATTGAAGAGAGAGAGCGGCGGCATCAGCGGA
TTTTGAAAGGTCTTGCTGATGTCGATGCGGGAAGAGTAGTAAGCCATGCAGATGTGATCGACTTCGCCAACAAACTGAAA
AAGTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZW91

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References