Detailed information of TA system TA192032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 119880..120439
• Replicon: plasmid unnamed1
• Accession: NZ_CP069883
• Organism: Escherichia coli strain FDAARGOS_1289

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: I6K19_RS25465
• Protein ID: WP_000421272.1
• Coordinates: 120164..120439 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: I6K19_RS25460
• Protein ID: WP_001387467.1
• Coordinates: 119880..120164 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K19_RS25435 (115343)	115343..115783	-	441	Protein_142	zinc-binding dehydrogenase	-
I6K19_RS25440 (116184)	116184..117074	+	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
I6K19_RS25445 (117099)	117099..117479	+	381	WP_000710783.1	Rid family detoxifying hydrolase	-
I6K19_RS25450 (117512)	117512..118477	+	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
I6K19_RS25455 (118523)	118523..119275	-	753	WP_000562172.1	IclR family transcriptional regulator	-
I6K19_RS25460 (119880)	119880..120164	+	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6K19_RS25465 (120164)	120164..120439	+	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K19_RS25470 (120545)	120545..120748	+	204	Protein_149	hypothetical protein	-
I6K19_RS25475 (120873)	120873..121001	-	129	Protein_150	IS481 family transposase	-
I6K19_RS25480 (121034)	121034..122203	-	1170	WP_001072355.1	hypothetical protein	-
I6K19_RS27250 (122300)	122300..122572	+	273	Protein_152	type II toxin-antitoxin system RelE/ParE family toxin	-
I6K19_RS25485 (122639)	122639..122821	+	183	WP_042344623.1	hypothetical protein	-
I6K19_RS25490 (122942)	122942..123682	+	741	WP_001066953.1	tyrosine-type recombinase/integrase	-
I6K19_RS25495 (123967)	123967..124944	-	978	WP_000361611.1	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / dfrA8 / tet(B)	-	1..131034	131034

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T192032 WP_000421272.1 NZ_CP069883:120164-120439 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T192032 NZ_CP092039:c4424462-4424157 [Pectobacterium brasiliense]
ATGCCACAAGTGATTGTTTCAGAACGTGCAAGACGTGATTTGCAACGTCTGCAGGATTTTCTAAAAAACAAAAATGCGCT
GGCTGCGAAAAAGGCGGCTGAAGTTTTGATTCGGGGCATTCGTCAGTTAGAAGCGCTGCCGAATATCGGGCGACCCGTTG
AACATCTCCCTCTTGAATATCAGGAACTGGTGATCGAATTCGGCAGCAGCGGGTACGTGGTGCTTTACCGCCATGATGAA
CTGACTGACAGAGTGGTGATCCTGACCGTCAAGCATCAAAAAGAAGCCGGTTACCAAAACCCTTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT192032 WP_001387467.1 NZ_CP069883:119880-120164 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT192032 NZ_CP092039:c4424740-4424450 [Pectobacterium brasiliense]
ATGGCTGACACGCCACGCCAAACCACGACGCTGCGCATCGATCAGGACATTAAAGAACGACTGAAAACGTTGGCCGATGA
CCGCCATAGCTCAGCCCATGCGCTAATGCTGGAAGCCATCACCGAATACGTGGAGCGTGAAGAAAAACGCAGTCAGTACC
GTAAAGAGGCGCTGGCCGCGTGGGAGGAATACCAGAGCACCGGCGAACACATCACCGCCGATGAGACGGTCACCTGGTTA
GAATCCTGGGGTTCTGGGCATGAACAGGATGCGCCTGCATGCCACAAGTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880