Detailed information of TA system TA192029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 11293..11818
• Replicon: plasmid unnamed1
• Accession: NZ_CP069883
• Organism: Escherichia coli strain FDAARGOS_1289

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: I6K19_RS24795
• Protein ID: WP_001159868.1
• Coordinates: 11513..11818 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: I6K19_RS24790
• Protein ID: WP_000813634.1
• Coordinates: 11293..11511 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K19_RS24765 (6406)	6406..7977	-	1572	WP_000381395.1	IS66-like element ISCro1 family transposase	-
I6K19_RS24770 (7997)	7997..8344	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
I6K19_RS24775 (8344)	8344..9021	-	678	WP_001299364.1	IS66-like element accessory protein TnpA	-
I6K19_RS24780 (9062)	9062..10180	+	1119	WP_204606457.1	hypothetical protein	-
I6K19_RS24785 (10214)	10214..10702	-	489	WP_011254646.1	hypothetical protein	-
I6K19_RS24790 (11293)	11293..11511	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
I6K19_RS24795 (11513)	11513..11818	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
I6K19_RS24800 (11819)	11819..12625	+	807	WP_000016982.1	site-specific integrase	-
I6K19_RS24805 (13399)	13399..14154	+	756	WP_000852146.1	replication initiation protein RepE	-
I6K19_RS24810 (14393)	14393..15043	+	651	WP_000993931.1	IS66-like element accessory protein TnpA	-
I6K19_RS24815 (15043)	15043..15390	+	348	WP_097455558.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / dfrA8 / tet(B)	-	1..131034	131034

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T192029 WP_001159868.1 NZ_CP069883:11513-11818 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T192029 NZ_CP092039:c4197258-4196884 [Pectobacterium brasiliense]
ATGCAGCTGTTATCATTGGTTGTGCATGGCCTATTAGTATTGCTCATTCTGCTGGCACCGTGGCCGGACGGCTATGCATG
GCTATGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTATCCGTAGCCAACGGAATATTAAATCCCGACAGGGGGAAA
TCGTTCTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAGGAATGGCAGATCGTTAAACGACCGTGGCTGCTGAAGAAT
GGCATATTACTGTCATTGCAAGCGGTTAACGGTAAAGACAAGCAACAGCTGTGGCTGGCATCGGACAGCATGGGTGATGA
CGAATGGCGTCACTTGCGTCAAATTCTTTTGCAGCAGAAACACTGGGCAAGATAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT192029 WP_000813634.1 NZ_CP069883:11293-11511 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT192029 NZ_CP092039:c4197544-4197278 [Pectobacterium brasiliense]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAATTGGATATCGCAATCATGCCGTT
TTTTGAGCATGACTATGACACACTTAATGACACTGATAAGCGCACGTTTGTACGCTTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGTGAACCGGAGGACGGAGAGCTGAAACGCATGATTTCCCTTATTCAGACGCGAAAT
AAAGATCGTGGCCCAGTGGCGATGTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2