Detailed information of TA system TA192017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3573729..3575317
• Replicon: chromosome
• Accession: NZ_CP069882
• Organism: Escherichia coli strain FDAARGOS_1289

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: I6K19_RS17025
• Protein ID: WP_001546825.1
• Coordinates: 3573729..3575051 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6K19_RS17030
• Protein ID: WP_001296726.1
• Coordinates: 3575051..3575317 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K19_RS16985 (3568763)	3568763..3569335	-	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-
I6K19_RS16990 (3569486)	3569486..3569638	-	153	WP_001322417.1	amidohydrolase family protein	-
I6K19_RS16995 (3569628)	3569628..3570047	-	420	WP_001322416.1	amidohydrolase family protein	-
I6K19_RS17000 (3570007)	3570007..3570162	-	156	WP_001322415.1	hypothetical protein	-
I6K19_RS17005 (3570265)	3570265..3571212	-	948	WP_204606429.1	aldo/keto reductase	-
I6K19_RS17010 (3571286)	3571286..3571975	-	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
I6K19_RS17015 (3571984)	3571984..3572292	-	309	WP_001221046.1	hypothetical protein	-
I6K19_RS17020 (3572432)	3572432..3573331	+	900	WP_001057776.1	LysR family transcriptional regulator	-
I6K19_RS17025 (3573729)	3573729..3575051	-	1323	WP_001546825.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6K19_RS17030 (3575051)	3575051..3575317	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49331.00 Da
• Isoelectric Point: 8.1369

>T192017 WP_001546825.1 NZ_CP069882:c3575051-3573729 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHCRLSREYGIK

Nucleotide

• Length: 1323 bp

>T192017 NZ_CP092037:4110341-4110691 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT192017 WP_001296726.1 NZ_CP069882:c3575317-3575051 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT192017 NZ_CP092037:4110096-4110347 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA