Detailed information of TA system TA192010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3127244..3128028
• Replicon: chromosome
• Accession: NZ_CP069882
• Organism: Escherichia coli strain FDAARGOS_1289

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: I6K19_RS14775
• Protein ID: WP_000613626.1
• Coordinates: 3127244..3127738 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: I6K19_RS14780
• Protein ID: WP_001110447.1
• Coordinates: 3127735..3128028 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K19_RS14755 (3122437)	3122437..3123534	+	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-
I6K19_RS14760 (3123534)	3123534..3124475	+	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I6K19_RS14765 (3124541)	3124541..3126184	+	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
I6K19_RS14770 (3126196)	3126196..3127149	+	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
I6K19_RS14775 (3127244)	3127244..3127738	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
I6K19_RS14780 (3127735)	3127735..3128028	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
I6K19_RS14785 (3128161)	3128161..3131346	-	3186	WP_000827427.1	ribonuclease E	-
I6K19_RS14790 (3131919)	3131919..3132878	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T192010 WP_000613626.1 NZ_CP069882:c3127738-3127244 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T192010 NZ_CP092037:c2625685-2625350 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT192010 WP_001110447.1 NZ_CP069882:c3128028-3127735 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT192010 NZ_CP092037:c2625933-2625685 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6