Detailed information of TA system TA192009

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2932374..2933169
• Replicon: chromosome
• Accession: NZ_CP069882
• Organism: Escherichia coli strain FDAARGOS_1289

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: I6K19_RS13850
• Protein ID: WP_000854914.1
• Coordinates: 2932795..2933169 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6K19_RS13845
• Protein ID: WP_204606420.1
• Coordinates: 2932374..2932748 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K19_RS13810 (2928624)	2928624..2929078	+	455	Protein_2719	IrmA family protein	-
I6K19_RS13815 (2929157)	2929157..2929390	+	234	WP_001119717.1	DUF905 family protein	-
I6K19_RS13820 (2929490)	2929490..2930308	+	819	WP_001234726.1	DUF932 domain-containing protein	-
I6K19_RS13825 (2930400)	2930400..2930885	+	486	WP_000214307.1	antirestriction protein	-
I6K19_RS13830 (2930901)	2930901..2931377	+	477	WP_001186726.1	RadC family protein	-
I6K19_RS13835 (2931440)	2931440..2931661	+	222	WP_000692298.1	DUF987 domain-containing protein	-
I6K19_RS13840 (2931680)	2931680..2932363	+	684	WP_000086768.1	hypothetical protein	-
I6K19_RS13845 (2932374)	2932374..2932748	+	375	WP_204606420.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
I6K19_RS13850 (2932795)	2932795..2933169	+	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
I6K19_RS13855 (2933166)	2933166..2933657	+	492	WP_204606421.1	DUF5983 family protein	-
I6K19_RS13860 (2933674)	2933674..2933871	+	198	WP_000839253.1	DUF957 domain-containing protein	-
I6K19_RS13865 (2933956)	2933956..2934516	+	561	Protein_2730	DUF4942 domain-containing protein	-
I6K19_RS13870 (2934588)	2934588..2935592	+	1005	WP_001179707.1	IS110-like element ISSfl8 family transposase	-
I6K19_RS13875 (2935874)	2935874..2936155	+	282	Protein_2732	DUF4942 domain-containing protein	-
I6K19_RS13885 (2936646)	2936646..2936864	-	219	WP_001040187.1	translation initiation factor IF-1	-
I6K19_RS13890 (2937149)	2937149..2937853	-	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2934588..2935592	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T192009 WP_000854914.1 NZ_CP069882:2932795-2933169 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T192009 NZ_CP092037:2493710-2494783 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13650.47 Da
• Isoelectric Point: 5.5686

>AT192009 WP_204606420.1 NZ_CP069882:2932374-2932748 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT192009 NZ_CP092037:2494776-2495147 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5