Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pmenTA/darT(toxin) |
| Location | 2907273..2908993 | Replicon | chromosome |
| Accession | NZ_CP069882 | ||
| Organism | Escherichia coli strain FDAARGOS_1289 | ||
Toxin (Protein)
| Gene name | pmenT | Uniprot ID | A0A7U9J2A7 |
| Locus tag | I6K19_RS13760 | Protein ID | WP_000312833.1 |
| Coordinates | 2908337..2908993 (-) | Length | 219 a.a. |
Antitoxin (Protein)
| Gene name | pmenA | Uniprot ID | A0A8B5IZW9 |
| Locus tag | I6K19_RS13755 | Protein ID | WP_032218015.1 |
| Coordinates | 2907273..2908340 (-) | Length | 356 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I6K19_RS13740 (2902484) | 2902484..2903098 | - | 615 | Protein_2705 | IS3-like element IS600 family transposase | - |
| I6K19_RS13745 (2903443) | 2903443..2904159 | - | 717 | WP_001122107.1 | SprT family zinc-dependent metalloprotease | - |
| I6K19_RS13750 (2904159) | 2904159..2907254 | - | 3096 | WP_204606419.1 | HsdR family type I site-specific deoxyribonuclease | - |
| I6K19_RS13755 (2907273) | 2907273..2908340 | - | 1068 | WP_032218015.1 | toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG | Antitoxin |
| I6K19_RS13760 (2908337) | 2908337..2908993 | - | 657 | WP_000312833.1 | toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT | Toxin |
| I6K19_RS13765 (2909006) | 2909006..2910361 | - | 1356 | WP_223349791.1 | restriction endonuclease subunit S | - |
| I6K19_RS13770 (2910361) | 2910361..2912799 | - | 2439 | WP_032180025.1 | type I restriction-modification system subunit M | - |
| I6K19_RS13775 (2912839) | 2912839..2913078 | - | 240 | WP_024224214.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | iroN / iroE / iroE / iroD / iroC / aggD / afaC-I | 2875313..2908993 | 33680 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 219 a.a. Molecular weight: 25233.56 Da Isoelectric Point: 6.2145
>T192008 WP_000312833.1 NZ_CP069882:c2908993-2908337 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS
Download Length: 657 bp
>T192008 NZ_CP092037:c1870827-1870576 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA
Antitoxin
Download Length: 356 a.a. Molecular weight: 40688.74 Da Isoelectric Point: 5.6927
>AT192008 WP_032218015.1 NZ_CP069882:c2908340-2907273 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQLLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQLLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC
Download Length: 1068 bp
>AT192008 NZ_CP092037:c1870579-1870325 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9J2A7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A8B5IZW9 |