Detailed information of TA system TA192007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2789305..2790010
• Replicon: chromosome
• Accession: NZ_CP069882
• Organism: Escherichia coli strain FDAARGOS_1289

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I6K19_RS13215
• Protein ID: WP_000539521.1
• Coordinates: 2789624..2790010 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6K19_RS13210
• Protein ID: WP_001280945.1
• Coordinates: 2789305..2789634 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K19_RS13195 (2784462)	2784462..2785373	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
I6K19_RS13200 (2785551)	2785551..2787899	+	2349	WP_001322378.1	EAL domain-containing protein	-
I6K19_RS13205 (2787907)	2787907..2789235	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
I6K19_RS13210 (2789305)	2789305..2789634	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I6K19_RS13215 (2789624)	2789624..2790010	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K19_RS13220 (2790236)	2790236..2791561	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6K19_RS13225 (2791774)	2791774..2792157	+	384	WP_000497137.1	biofilm formation regulator BssR	-
I6K19_RS13230 (2792268)	2792268..2793383	+	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
I6K19_RS13235 (2793380)	2793380..2794006	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T192007 WP_000539521.1 NZ_CP069882:c2790010-2789624 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T192007 NZ_CP092037:c1870579-1870325 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT192007 WP_001280945.1 NZ_CP069882:c2789634-2789305 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT192007 NZ_CP092037:c1870827-1870576 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6