Detailed information of TA system TA191998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 12579..13315
• Replicon: plasmid unnamed1
• Accession: NZ_CP069875
• Organism: Klebsiella pneumoniae strain FDAARGOS_1323

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: I6K53_RS25750
• Protein ID: WP_003026803.1
• Coordinates: 12833..13315 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I6K53_RS25745
• Protein ID: WP_003026799.1
• Coordinates: 12579..12845 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K53_RS25700	8641..9003	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
I6K53_RS25705	9053..9403	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
I6K53_RS25710	9761..10030	+	270	WP_004152102.1	hypothetical protein	-
I6K53_RS25715	10018..10593	+	576	WP_004152103.1	hypothetical protein	-
I6K53_RS25720	10624..11118	+	495	WP_004152104.1	DNA-binding protein	-
I6K53_RS25725	11162..11530	+	369	WP_004152105.1	hypothetical protein	-
I6K53_RS25730	11564..11767	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
I6K53_RS25735	11816..12073	+	258	WP_004152107.1	hypothetical protein	-
I6K53_RS25740	12149..12403	+	255	WP_004152108.1	hypothetical protein	-
I6K53_RS25745	12579..12845	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I6K53_RS25750	12833..13315	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
I6K53_RS25755	13523..14869	+	1347	WP_077253535.1	ISNCY family transposase	-
I6K53_RS25760	14918..15313	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
I6K53_RS25765	15461..16715	-	1255	Protein_23	IS3 family transposase	-
I6K53_RS25770	16803..17765	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aac(3)-IIa / dfrA14 / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A) / qnrB1	-	1..141542	141542

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T191998 WP_003026803.1 NZ_CP069875:12833-13315 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T191998 NZ_CP092037:c437912-437694 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT191998 WP_003026799.1 NZ_CP069875:12579-12845 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT191998 NZ_CP092037:c438312-437938 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66