Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2961735..2962354 Replicon chromosome
Accession NZ_CP069873
Organism Klebsiella pneumoniae strain FDAARGOS_1323

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I6K53_RS14250 Protein ID WP_002892050.1
Coordinates 2961735..2961953 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag I6K53_RS14255 Protein ID WP_002892066.1
Coordinates 2961980..2962354 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K53_RS14215 2957782..2958045 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
I6K53_RS14220 2958045..2958185 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I6K53_RS14225 2958182..2958880 - 699 WP_032435564.1 GNAT family N-acetyltransferase -
I6K53_RS14230 2958981..2960432 + 1452 WP_032435563.1 PLP-dependent aminotransferase family protein -
I6K53_RS14235 2960407..2960877 - 471 WP_002892026.1 YlaC family protein -
I6K53_RS14240 2960898..2961038 + 141 WP_004147370.1 hypothetical protein -
I6K53_RS14245 2961010..2961576 - 567 WP_002892030.1 maltose O-acetyltransferase -
I6K53_RS14250 2961735..2961953 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I6K53_RS14255 2961980..2962354 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
I6K53_RS14260 2962840..2965986 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
I6K53_RS14265 2966009..2967202 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T191987 WP_002892050.1 NZ_CP069873:c2961953-2961735 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T191987 NZ_CP092014:c3217984-3217703 [Edwardsiella ictaluri]
ATGACCTATAAACTGAGCTTTGAAAAGCGAGCCCTGAAAGAGTGGAAAAAACTGGCACCGCCGATTCAAAGCCAGTTGAA
AAAGAAACTGATCGAACGCCTGGAAAACCCACATGTTCCAGCAGCACGCTTAAGTGGGCGCGCTAACCGTTATAAAATAA
AATTACGCTCTTCCGGTTACCGGTTAGTTTATGAAGTCAACGATAGCGAAATCATCCTGCTGGTGATCGCTATCGGTAAA
CGGGCCGATAACGAGGTGTACCAAGCGGCAGACAGTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT191987 WP_002892066.1 NZ_CP069873:c2962354-2961980 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT191987 NZ_CP092014:c3218225-3217974 [Edwardsiella ictaluri]
ATGGCATACCAAATTCTCACGACCACCGCCGCCAGCATTACCGACCTGAAACGCAATCCAATGGGTACCATTGCTGAAGG
TGACGGCAATGCCGTAGCAATCTTAAACCGGAATGAGCCGGCCTTTTACTGCGTTCCACCGGAACTCTACGCTTACTATC
TGGAGCTGGCTGAAGATGCGGCGCTTAATCGTATCGCCGATGAGCGTCTGGAGGACGCTGAGTTTGTCAGCGTAAATATC
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References