Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2319838..2320648 Replicon chromosome
Accession NZ_CP069873
Organism Klebsiella pneumoniae strain FDAARGOS_1323

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag I6K53_RS11170 Protein ID WP_004178461.1
Coordinates 2320115..2320648 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag I6K53_RS11165 Protein ID WP_002887278.1
Coordinates 2319838..2320104 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K53_RS11145 2315709..2316053 - 345 WP_004894569.1 cation efflux system protein CusF -
I6K53_RS11150 2316071..2317456 - 1386 WP_026005908.1 efflux transporter outer membrane subunit -
I6K53_RS11155 2317629..2318312 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
I6K53_RS11160 2318302..2319735 + 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
I6K53_RS11165 2319838..2320104 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
I6K53_RS11170 2320115..2320648 + 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
I6K53_RS11175 2320696..2321817 - 1122 WP_004186850.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T191986 WP_004178461.1 NZ_CP069873:2320115-2320648 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T191986 NZ_CP092014:c3206679-3206350 [Edwardsiella ictaluri]
ATGAACTACACTATTGAGTACTACAGCGAAGAGGTTCGGCTTGAGGTCGATCAGTTGCCAATGGGTATGCGGGTTCGATA
CCAGCATCTCGTTGAACGCATGGAAATCTACGGTAGTAATCTCGGAGAACCACACACAAGCCCTTTTGGCGATGGGCTTT
TTGAGCTTCGAATCAAAGGCAGTGATGGCATCGCCCGCGTGTTTTACTGCACTCTTACCGGAAAACGTATCGTCATGCTG
CACAGCTTCATCAAGAAAACCCAGAAGACGCCAAGCGCTGAACGTAAGAAGGCTGAAACCAGAATGAAGGAGGTAAAACA
TGGCTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT191986 WP_002887278.1 NZ_CP069873:2319838-2320104 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT191986 NZ_CP092014:c3206334-3206053 [Edwardsiella ictaluri]
ATGACGCATGATGAAATGGCTGCAAAATGGATGGAAGACCCGGCATTTAAAGCAGAATACGATGCCATCGAAGAGGAATA
CGCCCTACTCGATGAAATGCTCGCAGCCCGCAAAAACGCGGGGCTGACCCAGGCGCAGGTCGCCGAACGTATGGGAACCA
AGGCTACAGCGATCACTCGCATGGAAAGTAATTTAGCCTCCGGCCAAAGCGGGCCATCATTCGCCACGCTGAAAAAATTT
GCACACGCGACCGGCAAGAAACTGCAGATCCGGTTTGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References