Detailed information of TA system TA191978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 66475..67034
• Replicon: plasmid unnamed3
• Accession: NZ_CP069867
• Organism: Escherichia coli strain FDAARGOS_1282

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: I6K12_RS25330
• Protein ID: WP_000421257.1
• Coordinates: 66759..67034 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: I6K12_RS25325
• Protein ID: WP_001178089.1
• Coordinates: 66475..66759 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K12_RS25305 (I6K12_25310)	61504..62124	-	621	WP_000176498.1	DUF1449 family protein	-
I6K12_RS25310 (I6K12_25315)	62391..63053	-	663	WP_000154142.1	hypothetical protein	-
I6K12_RS25315 (I6K12_25320)	63194..63835	-	642	WP_000335849.1	transcription termination/antitermination NusG family protein	-
I6K12_RS25320 (I6K12_25325)	64536..65567	+	1032	WP_000907880.1	plasmid replication initiator RepA	-
I6K12_RS25325 (I6K12_25330)	66475..66759	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6K12_RS25330 (I6K12_25335)	66759..67034	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K12_RS25335 (I6K12_25340)	67140..67343	+	204	Protein_71	hypothetical protein	-
I6K12_RS25340 (I6K12_25345)	67612..67878	-	267	WP_000627831.1	hypothetical protein	-
I6K12_RS25350 (I6K12_25355)	68116..68718	-	603	WP_000517694.1	ProQ/FINO family protein	-
I6K12_RS25355 (I6K12_25360)	69072..70418	+	1347	WP_001132021.1	protein YagA	-
I6K12_RS25360 (I6K12_25365)	70937..71239	-	303	WP_001245884.1	hypothetical protein	-
I6K12_RS25365 (I6K12_25370)	71236..71862	-	627	WP_000587689.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..81604	81604

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T191978 WP_000421257.1 NZ_CP069867:66759-67034 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T191978 NZ_CP092012:c4326372-4325881 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGAACCATCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT191978 WP_001178089.1 NZ_CP069867:66475-66759 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT191978 NZ_CP092012:c4326662-4326369 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCACAGGCTTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A731IHA8