Detailed information of TA system TA191976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4638376..4639014
• Replicon: chromosome
• Accession: NZ_CP069865
• Organism: Escherichia coli strain FDAARGOS_1282

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: I6K12_RS22735
• Protein ID: WP_001306887.1
• Coordinates: 4638376..4638552 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6K12_RS22740
• Protein ID: WP_001270286.1
• Coordinates: 4638598..4639014 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K12_RS22710 (4633432)	4633432..4633890	+	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-
I6K12_RS22715 (4633998)	4633998..4635209	-	1212	WP_023143671.1	BenE family transporter YdcO	-
I6K12_RS22720 (4635262)	4635262..4635798	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
I6K12_RS22725 (4635871)	4635871..4637832	+	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I6K12_RS22730 (4637924)	4637924..4638154	-	231	WP_000494241.1	YncJ family protein	-
I6K12_RS22735 (4638376)	4638376..4638552	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I6K12_RS22740 (4638598)	4638598..4639014	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I6K12_RS22745 (4639093)	4639093..4640499	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
I6K12_RS22750 (4640744)	4640744..4641889	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
I6K12_RS22755 (4641907)	4641907..4642920	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
I6K12_RS22760 (4642921)	4642921..4643862	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4633432..4633890	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T191976 WP_001306887.1 NZ_CP069865:4638376-4638552 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T191976 NZ_CP092012:3654495-3654752 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAATGCCTCCGGCGCCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCAGTTACTCACATATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAACAGCTTTACAAGGCTGAACAGGTGGTTAAGGGGATG
CGGGACGTGATTGTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT191976 WP_001270286.1 NZ_CP069865:4638598-4639014 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT191976 NZ_CP092012:c3654670-3654338 [Salmonella enterica subsp. enterica serovar Kentucky]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCTGCCA
GTCACCCTTAAGGTGCTGGCTGGGGATATGTGAGTAACTGGTGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGG
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTTAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGTACCGGGCGGTGATGAGCAACCGGCTACC
GGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: A0A0D8WF76