Detailed information of TA system TA191969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4151119..4151903
• Replicon: chromosome
• Accession: NZ_CP069865
• Organism: Escherichia coli strain FDAARGOS_1282

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: I6K12_RS20150
• Protein ID: WP_000613626.1
• Coordinates: 4151119..4151613 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: I6K12_RS20155
• Protein ID: WP_001110447.1
• Coordinates: 4151610..4151903 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K12_RS20130 (4146312)	4146312..4147409	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
I6K12_RS20135 (4147409)	4147409..4148350	+	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I6K12_RS20140 (4148416)	4148416..4150059	+	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
I6K12_RS20145 (4150071)	4150071..4151024	+	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
I6K12_RS20150 (4151119)	4151119..4151613	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
I6K12_RS20155 (4151610)	4151610..4151903	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
I6K12_RS20160 (4152036)	4152036..4155221	-	3186	WP_001522867.1	ribonuclease E	-
I6K12_RS20165 (4155794)	4155794..4156753	+	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T191969 WP_000613626.1 NZ_CP069865:c4151613-4151119 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T191969 NZ_CP092012:c95656-95138 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGGACAACCTGACAATAGAGATTCTGGCTGATAACGCAGAATACAACTGGCGGCAGTTCGACTGCGGTGAGGCATCATT
AAACCTTTTTTTAACGCAGCATCTTCAGCGCCAGCATAACAACAAAATCTTGCGCGGTTATGTACTCAGAACCACTACGC
CAGAACGGCGAGTGTTGGGGTATTACACCCTGTCAGGAAGCTGCTTTGAACGCGCGTCGTTGCCTTCCAGAACCCAGCAA
AAGCGCATCCCTTACCAGAATATCCCCAGCGTTACGCTGGGCCGTTTAGCGGTCGATCTCTCATTACAGGGAAAAGGGTG
GGGAGCTATATTGGTCACGCATGCGATGAAAGTGGTGTGGTCGGCATCACTGGCCGTTGGGATTCATGGCATCTTTGTGG
AAGCGTTAAACGAAAAAGCCCAGGCCTTTTATCAGCGGCTGGGCTTTATTTCTTTATCCGGAGAAAACGAGCACGCGCTG
TTTTACCCGACAAAGTCGATTGAGCAACTTTTTGGCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT191969 WP_001110447.1 NZ_CP069865:c4151903-4151610 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT191969 NZ_CP092012:c95929-95660 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCAGCCCTAAAAAAACAGCGTATCGATCTGCGGCTCACAGACGGCGATAAAAGCATGATTGAAGAAGCGGCCGCCAT
TACTAATCAATCAATCACGCAATTTATGTTGAATAGCGCAGCGGAGCGAGCCGCAGAAGTGCTTGAACAGCACCGTCGGG
TTATTCTTAATGAAGCATCCTGGGCGCGAGTCATGGATGCGCTTAGTCATCCTCCCACCCCCAATGAGAAATTAAAACGC
GCGGCGCAACGTCTACAAGACATGGAGTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6