Detailed information of TA system TA191968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3895597..3896302
• Replicon: chromosome
• Accession: NZ_CP069865
• Organism: Escherichia coli strain FDAARGOS_1282

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I6K12_RS18950
• Protein ID: WP_000539521.1
• Coordinates: 3895916..3896302 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6K12_RS18945
• Protein ID: WP_001280945.1
• Coordinates: 3895597..3895926 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K12_RS18930 (3890754)	3890754..3891665	+	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-
I6K12_RS18935 (3891843)	3891843..3894191	+	2349	WP_021523051.1	EAL domain-containing protein	-
I6K12_RS18940 (3894199)	3894199..3895527	+	1329	Protein_3570	diguanylate cyclase	-
I6K12_RS18945 (3895597)	3895597..3895926	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I6K12_RS18950 (3895916)	3895916..3896302	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K12_RS18955 (3896528)	3896528..3897853	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6K12_RS18960 (3898066)	3898066..3898449	+	384	WP_000497137.1	biofilm formation regulator BssR	-
I6K12_RS18965 (3898560)	3898560..3899675	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
I6K12_RS18970 (3899672)	3899672..3900298	-	627	WP_020233670.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T191968 WP_000539521.1 NZ_CP069865:c3896302-3895916 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T191968 NZ_CP092009:4609711-4610022 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT191968 WP_001280945.1 NZ_CP069865:c3895926-3895597 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT191968 NZ_CP092009:4610019-4610465 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6