Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3473605..3474223 Replicon chromosome
Accession NZ_CP069865
Organism Escherichia coli strain FDAARGOS_1282

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K12_RS17095 Protein ID WP_001291435.1
Coordinates 3473605..3473823 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K12_RS17100 Protein ID WP_000344800.1
Coordinates 3473849..3474223 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K12_RS17060 (3468894) 3468894..3469466 + 573 WP_000779831.1 YbaY family lipoprotein -
I6K12_RS17065 (3469497) 3469497..3469808 - 312 WP_000409911.1 MGMT family protein -
I6K12_RS17075 (3470187) 3470187..3470540 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K12_RS17080 (3470582) 3470582..3472132 - 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K12_RS17085 (3472296) 3472296..3472766 - 471 WP_001490888.1 YlaC family protein -
I6K12_RS17090 (3472882) 3472882..3473433 - 552 WP_001522034.1 maltose O-acetyltransferase -
I6K12_RS17095 (3473605) 3473605..3473823 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K12_RS17100 (3473849) 3473849..3474223 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K12_RS17105 (3474769) 3474769..3477918 - 3150 WP_204667180.1 multidrug efflux RND transporter permease subunit AcrB -
I6K12_RS17110 (3477941) 3477941..3479134 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T191967 WP_001291435.1 NZ_CP069865:c3473823-3473605 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T191967 NZ_CP092009:c4608478-4608167 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT191967 WP_000344800.1 NZ_CP069865:c3474223-3473849 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT191967 NZ_CP092009:c4608166-4607876 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTAAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGCTCACCGACAGGGCCAGCCAAAGCATTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References