Detailed information of TA system TA191966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3267811..3269500
• Replicon: chromosome
• Accession: NZ_CP069865
• Organism: Escherichia coli strain FDAARGOS_1282

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A829L165
• Locus tag: I6K12_RS16140
• Protein ID: WP_001521912.1
• Coordinates: 3268580..3269500 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: I6K12_RS16135
• Protein ID: WP_001521911.1
• Coordinates: 3267811..3268593 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K12_RS16110 (3263120)	3263120..3263278	-	159	Protein_3021	integrase	-
I6K12_RS16115 (3263370)	3263370..3263472	-	103	Protein_3022	helix-turn-helix domain-containing protein	-
I6K12_RS16120 (3263469)	3263469..3263939	-	471	WP_000201185.1	hypothetical protein	-
I6K12_RS16130 (3264286)	3264286..3267495	-	3210	WP_001521910.1	RNA-directed DNA polymerase	-
I6K12_RS16135 (3267811)	3267811..3268593	+	783	WP_001521911.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
I6K12_RS16140 (3268580)	3268580..3269500	+	921	WP_001521912.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
I6K12_RS16145 (3269934)	3269934..3270818	-	885	WP_001119037.1	LysR family transcriptional regulator	-
I6K12_RS16150 (3271083)	3271083..3272141	+	1059	WP_122452191.1	alpha/beta hydrolase	-
I6K12_RS16155 (3272283)	3272283..3273410	+	1128	WP_001521913.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34601.04 Da
• Isoelectric Point: 7.2649

>T191966 WP_001521912.1 NZ_CP069865:3268580-3269500 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T191966 NZ_CP092009:c4291790-4291299 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGAACCATCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29168.46 Da
• Isoelectric Point: 8.4458

>AT191966 WP_001521911.1 NZ_CP069865:3267811-3268593 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT191966 NZ_CP092009:c4292080-4291787 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCACAGGCTTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A829L165