Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2753382..2753904 Replicon chromosome
Accession NZ_CP069865
Organism Escherichia coli strain FDAARGOS_1282

Toxin (Protein)


Gene name relE Uniprot ID A0A829L6G8
Locus tag I6K12_RS13705 Protein ID WP_001105433.1
Coordinates 2753614..2753904 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SC69
Locus tag I6K12_RS13700 Protein ID WP_000212715.1
Coordinates 2753382..2753624 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K12_RS13680 (2749098) 2749098..2750471 + 1374 WP_001522402.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -
I6K12_RS13685 (2750623) 2750623..2751174 - 552 WP_000166270.1 ribosome biogenesis factor YjgA -
I6K12_RS13690 (2751268) 2751268..2752620 + 1353 WP_001162173.1 metalloprotease PmbA -
I6K12_RS13695 (2752804) 2752804..2753190 + 387 WP_001232246.1 cytochrome b562 -
I6K12_RS13700 (2753382) 2753382..2753624 + 243 WP_000212715.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
I6K12_RS13705 (2753614) 2753614..2753904 + 291 WP_001105433.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K12_RS13710 (2753905) 2753905..2754369 - 465 WP_001009182.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
I6K12_RS13715 (2754554) 2754554..2756692 - 2139 WP_000187798.1 anaerobic ribonucleoside-triphosphate reductase -
I6K12_RS13720 (2757086) 2757086..2758741 - 1656 WP_021523332.1 alpha,alpha-phosphotrehalase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11367.23 Da        Isoelectric Point: 10.0238

>T191963 WP_001105433.1 NZ_CP069865:2753614-2753904 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Download         Length: 291 bp

>T191963 NZ_CP092009:3420601-3420819 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9125.56 Da        Isoelectric Point: 5.1369

>AT191963 WP_000212715.1 NZ_CP069865:2753382-2753624 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Download         Length: 243 bp

>AT191963 NZ_CP092009:3420199-3420573 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L6G8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9H4B3

References