Detailed information of TA system TA191962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2741768..2742363
• Replicon: chromosome
• Accession: NZ_CP069865
• Organism: Escherichia coli strain FDAARGOS_1282

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: I6K12_RS13645
• Protein ID: WP_000239581.1
• Coordinates: 2742013..2742363 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: I6K12_RS13640
• Protein ID: WP_001223213.1
• Coordinates: 2741768..2742019 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K12_RS13630 (2737433)	2737433..2741212	+	3780	WP_001522396.1	autotransporter assembly complex protein TamB	-
I6K12_RS13635 (2741215)	2741215..2741556	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
I6K12_RS13640 (2741768)	2741768..2742019	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
I6K12_RS13645 (2742013)	2742013..2742363	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
I6K12_RS13650 (2742442)	2742442..2742972	-	531	WP_000055075.1	inorganic diphosphatase	-
I6K12_RS13655 (2743282)	2743282..2744238	+	957	WP_001522398.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
I6K12_RS13660 (2744371)	2744371..2745873	+	1503	WP_001522399.1	sugar ABC transporter ATP-binding protein	-
I6K12_RS13665 (2745887)	2745887..2746909	+	1023	WP_001361374.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T191962 WP_000239581.1 NZ_CP069865:2742013-2742363 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T191962 NZ_CP092009:2429455-2429739 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT191962 WP_001223213.1 NZ_CP069865:2741768-2742019 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT191962 NZ_CP092009:2429217-2429465 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7