Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2644317..2644814 Replicon chromosome
Accession NZ_CP069865
Organism Escherichia coli strain FDAARGOS_1282

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K12_RS13140 Protein ID WP_001173343.1
Coordinates 2644641..2644814 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6K12_RS13135 Protein ID WP_000398619.1
Coordinates 2644317..2644613 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K12_RS13115 (2641046) 2641046..2641765 - 720 WP_000611288.1 two-component system response regulator DcuR -
I6K12_RS13120 (2641762) 2641762..2643393 - 1632 WP_001216477.1 sensor histidine kinase -
I6K12_RS13125 (2643574) 2643574..2643804 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
I6K12_RS13130 (2643816) 2643816..2644088 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6K12_RS13135 (2644317) 2644317..2644613 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K12_RS13140 (2644641) 2644641..2644814 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K12_RS13145 (2644933) 2644933..2646450 - 1518 WP_000003806.1 lysine--tRNA ligase -
I6K12_RS13150 (2646687) 2646687..2648144 - 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T191961 WP_001173343.1 NZ_CP069865:2644641-2644814 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T191961 NZ_CP092009:2062739-2062842 [Salmonella enterica subsp. enterica serovar Kentucky]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT191961 WP_000398619.1 NZ_CP069865:2644317-2644613 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT191961 NZ_CP092009:c2062844-2062701 [Salmonella enterica subsp. enterica serovar Kentucky]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References