Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 70007..70532 Replicon plasmid unnamed1
Accession NZ_CP069864
Organism Escherichia coli strain FDAARGOS_1282

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag I6K12_RS00445 Protein ID WP_001159871.1
Coordinates 70007..70312 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6K12_RS00450 Protein ID WP_000813634.1
Coordinates 70314..70532 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K12_RS00410 (65434) 65434..66231 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
I6K12_RS00415 (66293) 66293..66544 + 252 Protein_84 DUF4113 domain-containing protein -
I6K12_RS00420 (66549) 66549..66941 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
I6K12_RS00425 (66946) 66946..67917 - 972 WP_001103694.1 plasmid segregation protein ParM -
I6K12_RS00430 (68146) 68146..68790 + 645 WP_000633911.1 AAA family ATPase -
I6K12_RS00435 (68784) 68784..69059 + 276 WP_000239529.1 hypothetical protein -
I6K12_RS00440 (69197) 69197..70006 - 810 WP_000016979.1 site-specific integrase -
I6K12_RS00445 (70007) 70007..70312 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
I6K12_RS00450 (70314) 70314..70532 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6K12_RS00455 (72854) 72854..73495 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) senB 1..115574 115574


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T191945 WP_001159871.1 NZ_CP069864:c70312-70007 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T191945 NZ_CP092004:c67964-67437 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCATGGATTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGACACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCGGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT191945 WP_000813634.1 NZ_CP069864:c70532-70314 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT191945 NZ_CP092004:c68251-67961 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References