Detailed information of TA system TA191945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 70007..70532
• Replicon: plasmid unnamed1
• Accession: NZ_CP069864
• Organism: Escherichia coli strain FDAARGOS_1282

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: I6K12_RS00445
• Protein ID: WP_001159871.1
• Coordinates: 70007..70312 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: I6K12_RS00450
• Protein ID: WP_000813634.1
• Coordinates: 70314..70532 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K12_RS00410 (65434)	65434..66231	+	798	WP_000544830.1	IS21-like element IS21 family helper ATPase IstB	-
I6K12_RS00415 (66293)	66293..66544	+	252	Protein_84	DUF4113 domain-containing protein	-
I6K12_RS00420 (66549)	66549..66941	-	393	WP_000340835.1	plasmid partitioning/stability family protein	-
I6K12_RS00425 (66946)	66946..67917	-	972	WP_001103694.1	plasmid segregation protein ParM	-
I6K12_RS00430 (68146)	68146..68790	+	645	WP_000633911.1	AAA family ATPase	-
I6K12_RS00435 (68784)	68784..69059	+	276	WP_000239529.1	hypothetical protein	-
I6K12_RS00440 (69197)	69197..70006	-	810	WP_000016979.1	site-specific integrase	-
I6K12_RS00445 (70007)	70007..70312	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
I6K12_RS00450 (70314)	70314..70532	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
I6K12_RS00455 (72854)	72854..73495	+	642	WP_000990667.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	senB	1..115574	115574

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T191945 WP_001159871.1 NZ_CP069864:c70312-70007 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T191945 NZ_CP092004:c67964-67437 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCATGGATTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGACACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCGGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT191945 WP_000813634.1 NZ_CP069864:c70532-70314 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT191945 NZ_CP092004:c68251-67961 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A829CQY2