Detailed information of TA system TA191944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 199967..200703
• Replicon: plasmid unnamed2
• Accession: NZ_CP069863
• Organism: Klebsiella pneumoniae strain FDAARGOS_1311

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: I6K41_RS27195
• Protein ID: WP_023329018.1
• Coordinates: 200221..200703 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I6K41_RS27190
• Protein ID: WP_003026799.1
• Coordinates: 199967..200233 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K41_RS27165	195015..196409	+	1395	WP_032738443.1	cytosine permease	-
I6K41_RS27170	196421..197629	+	1209	WP_023329016.1	imidazolonepropionase	-
I6K41_RS27175	197622..198431	+	810	WP_023329017.1	N-formylglutamate deformylase	-
I6K41_RS27180	198583..198717	-	135	WP_109241503.1	integrase core domain-containing protein	-
I6K41_RS27185	198684..198863	-	180	Protein_206	transposase	-
I6K41_RS27190	199967..200233	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I6K41_RS27195	200221..200703	+	483	WP_023329018.1	GNAT family N-acetyltransferase	Toxin
I6K41_RS27200	200910..202253	+	1344	WP_077263966.1	ISNCY family transposase	-
I6K41_RS27205	202324..203142	-	819	WP_023307220.1	abortive infection family protein	-
I6K41_RS27210	203151..203723	-	573	WP_032738445.1	recombinase family protein	-
I6K41_RS27215	204011..204422	-	412	Protein_212	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..204513	204513
	-	flank	IS/Tn	-	-	200898..202253	1355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17255.84 Da
• Isoelectric Point: 7.8840

>T191944 WP_023329018.1 NZ_CP069863:200221-200703 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAEGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T191944 NZ_CP091999:4581434-4581745 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT191944 WP_003026799.1 NZ_CP069863:199967-200233 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT191944 NZ_CP091999:4581742-4582188 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66