Detailed information of TA system TA191943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 183206..183728
• Replicon: plasmid unnamed2
• Accession: NZ_CP069863
• Organism: Klebsiella pneumoniae strain FDAARGOS_1311

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: I6K41_RS27100
• Protein ID: WP_004187019.1
• Coordinates: 183206..183490 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: I6K41_RS27105
• Protein ID: WP_004187025.1
• Coordinates: 183480..183728 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K41_RS27075	178956..179643	+	688	Protein_184	DUF4113 domain-containing protein	-
I6K41_RS27080	179765..180208	+	444	WP_023329004.1	DUF2384 domain-containing protein	-
I6K41_RS27085	180205..180675	+	471	WP_023329005.1	RES family NAD+ phosphorylase	-
I6K41_RS27090	180786..181045	-	260	Protein_187	hypothetical protein	-
I6K41_RS27095	181847..183190	+	1344	WP_032738442.1	ISNCY family transposase	-
I6K41_RS27100	183206..183490	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K41_RS27105	183480..183728	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
I6K41_RS27110	184016..184984	+	969	WP_204745803.1	IS5 family transposase	-
I6K41_RS27115	185089..186456	-	1368	WP_023329010.1	formimidoylglutamate deiminase	-
I6K41_RS27120	186559..187320	+	762	WP_023329011.1	histidine utilization repressor	-
I6K41_RS27125	187317..187874	+	558	WP_023329012.1	HutD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..204513	204513
	-	flank	IS/Tn	-	-	184016..184984	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T191943 WP_004187019.1 NZ_CP069863:c183490-183206 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T191943 NZ_CP091999:c4580201-4579890 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT191943 WP_004187025.1 NZ_CP069863:c183728-183480 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT191943 NZ_CP091999:c4579889-4579599 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTAAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGCTCACCGACAGGGCCAGCCAAAGCATTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4